A different sort of Mott cell.
A different sort of Mott cell.
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另一种莫特细胞。
DOI：
10.1073/pnas.89.24.11688
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发表时间：
1992
影响因子：
11.1
通讯作者：
Wabl,M
中科院分区：
文献类型：
--
作者：
Jäck,HM;Beck-Engeser,G;Sloan,B;Wong,ML;Wabl,M
NYC是一种来源于B/W小鼠的B淋巴瘤细胞系。当NYC细胞与本身不产生免疫球蛋白的浆细胞瘤融合后，所产生的NYCH杂交瘤细胞是Mott细胞；即它们包含充满免疫球蛋白的大的细胞内小泡，即所谓的Russell小体。当NYCH.kappa，NYCH.kappa，一种已经失去产生重链能力的NYCH.kappa，被导入重链构建体时，只有当所转染的免疫球蛋白重链与NYC具有相同的可变区时，细胞内小泡中才会出现这种浓度的免疫球蛋白。此外，与传统的Mott细胞不同，杂交细胞以正常的速度分泌免疫球蛋白。
NYC is a B lymphoma cell line derived from B/W mice. Upon fusion of NYC cells with a plasmacytoma, which itself produces no immunoglobulin, the resulting NYCH hybridoma cells are Mott cells; i.e., they contain large intracellular vesicles filled with immunoglobulin, the so-called Russell bodies. When NYCH.kappa, a variant of NYCH that had lost the ability to produce heavy chain, was transfected with a heavy-chain construct, this concentration of immunoglobulin in the intracellular vesicles occurred only when the transfected immunoglobulin heavy chain had the same variable region as NYC. Moreover, unlike conventional Mott cells, the hybrid cells secrete immunoglobulin at a normal rate.