Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method.
Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method.
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DOI：
10.1007/978-1-0716-0655-1_16
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Shih HP
中科院分区：
文献类型：
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作者：
Maldonado M;Serrill JD;Shih HP
胰腺由不同的细胞群组成，被组织成不同的功能单位，包括腺泡团、朗格汉斯胰岛和导管系统。由于对糖尿病的研究，已经开发了几种光学技术来对胰岛群体进行三维可视化，以更好地了解其解剖特征。这些方法在很大程度上依赖于三维整体免疫荧光染色。在本章中，我们回顾了一种用于研究成人胰岛形态的改良的整体免疫荧光染色方法。这种方法使用较小的样本，并结合了封闭和渗透步骤。与现有的方法相比，这减少了所需的时间；该方法也与常规的共聚焦显微镜兼容。
The pancreas is composed of different cellular populations, organized into distinct functional units, including acinar clusters, islets of Langerhans, and the ductal system. As a result of research into diabetes, several optical techniques have been developed for the three-dimensional visualization of islet populations, so as to better understand their anatomical characteristics. These approaches are largely reliant on three-dimensional whole-mount immunofluorescence staining. In this chapter, we review a revised whole mount immunofluorescence staining method for studying adult pancreatic islet morphology. This method uses smaller samples and combines the blocking and permeabilization steps. This reduces the time needed, relative to existing protocols; the method is compatible with regular confocal microscopy as well.