Production and regulation of interleukin-11 by breast cancer cells
Production and regulation of interleukin-11 by breast cancer cells
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DOI：
10.1016/s0304-3835(97)00542-9
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发表时间：
1998-05-15
期刊：
影响因子：
9.7
通讯作者：
Body, JJ
中科院分区：
文献类型：
--
作者：
Lacroix, M;Siwek, B;Body, JJ
我们研究了13株乳腺癌细胞产生白介素11(IL-11)的情况。其中两个细胞株(MDA-MB-231和Hs578T)同时在蛋白质和mRNA水平表达细胞因子。II-II对所研究的5个BCC系的生长没有调节作用，包括两个产生细胞因子的BCC系。转化生长因子-β1能快速(2 H)和瞬时(24 H)诱导细胞产生IL-1，且呈剂量依赖关系，但对表皮生长因子、胰岛素样生长因子-I和-II、碱性成纤维细胞生长因子、血小板衍生生长因子和甲状旁腺激素无明显影响。环状AMP诱导剂Forskolin和蛋白激酶C的激活剂佛波醇12-肉豆蔻酸酯13-乙酸酯也刺激IL-11的产生。除II-II外，MDA-MB-231和Hs578T是仅有的产生IL-6蛋白和mRNA的BCC株。由于II-IL和IL-6是破骨细胞发育的强大刺激因子，而骨是转化生长因子-β(1)的主要来源，我们的数据表明II-IL和IL-6共同促进了MDA-MB-231细胞的高骨质破坏能力，并可能在乳腺癌诱导的骨溶解中发挥作用。爱思唯尔科学爱尔兰有限公司出版。
We have studied the production of interleukin-11 (Il-11) in 13 breast cancer cell (BCC) lines. Two of these cell lines (MDA-MB-231 and Hs578T) expressed the cytokine at both the protein and mRNA levels. Ii-II did not modulate the growth of five BCC lines examined, including the two cytokine-producing BCC lines. The production of Ii-Il was increased by transforming growth factor-pi in a dose-dependent manner with a rapid (2 h) and transient (24 h) mRNA induction, but not by epidermal growth factor, insulin-like growth factor-I and -II, basic fibroblast growth factor, platelet-derived growth factor or parathyroid hormone. The cyclic AMP inducer, forskolin, and the activator of protein kinase C, phorbol 12-myristate 13-acetate, also stimulated the production of Il-11. Besides Ii-II, MDA-MB-231 and Hs578T were the only BCC lines to produce interleukin-6 (Il-6) protein and mRNA. Since Ii-Il and Il-6 are potent stimulators of osteoclast development and bone is a major source of TGF-beta(1), our data suggest that Ii-Il, together with Il-6, contributes to the high bone destructive capacity of MDA-MB-231 cells and could play a role in breast cancer-induced osteolysis. Published by Elsevier Science Ireland Ltd.