Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020.

Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020.
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DOI：
10.2807/1560-7917.es.2021.26.3.2100008
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发表时间：
2021-01
期刊：
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
影响因子：
--
通讯作者：
COVID-Diagnosis HCL Study Group
COVID-Diagnosis HCL Study Group
中科院分区：
其他
文献类型：
--
作者：
Bal A;Destras G;Gaymard A;Stefic K;Marlet J;Eymieux S;Regue H;Semanas Q;d'Aubarede C;Billaud G;Laurent F;Gonzalez C;Mekki Y;Valette M;Bouscambert M;Gaudy-Graffin C;Lina B;Morfin F;Josset L;COVID-Diagnosis HCL Study Group
来源链接：
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848679/

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我们报告了导致在法国首次检测到令人担忧的202012/01(挥发性有机化合物)变异的战略(2020年12月21日)。首先，筛选在包括VOC在内的某些Δ-CoV-2变种中发现的尖峰(S)缺失H69-V70(ΔH69/SARS V70)。这种缺失与三靶点RT-PCRTaqPath试剂盒中的S基因靶向失败(SGTF)有关。随后，对SGTF样本进行全基因组测序。这种方法发现了与ΔH69/ΔV70共存的突变，包括S：N501Y。
We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69–V70 (ΔH69/ΔV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target failure (SGTF) in the three-target RT-PCR assay (TaqPath kit). Subsequently, SGTF samples are whole genome sequenced. This approach revealed mutations co-occurring with ΔH69/ΔV70 including S:N501Y in the VOC.