Expression of Pax-3 in the lateral neural plate is dependent on a Wnt-mediated signal from posterior nonaxial mesoderm
Expression of Pax-3 in the lateral neural plate is dependent on a Wnt-mediated signal from posterior nonaxial mesoderm
复制标题
DOI：
10.1006/dbio.1999.9319
复制
发表时间：
1999-08-15
影响因子：
2.7
通讯作者：
Kintner, C
中科院分区：
文献类型：
--
作者：
Bang, AG;Papalopulu, N;Kintner, C
在脊椎动物轴突的早期模式化过程中，配对结构域转录因子Pax-3的表达是通过从晚期组织者和后部非轴向中胚层发出的posteriorizing信号在后部神经板的侧部诱导的。使用显性阴性的方法，我们表明，在外植体试验中，Pax-3诱导活动的组织者不依赖于FGF，视黄酸或XWnt-8单独或组合，这表明组织者地图产生一个未知的后验因子。然而，Pax-3诱导信号从后非轴向中胚层是Wnt依赖的。我们发现，Pax-3的表达在外侧神经板扩大XWnt-8注射胚胎，并被显性负XWnt-8。同样，我们表明，同源结构域转录因子Msx-1，像Pax-3是一个早期标记的外侧神经板，诱导后非轴中胚层和显性负XWnt-8。最后，我们表明，Rohon-Beard初级神经元，一种在外侧神经板内发育的细胞类型，我们也在体内被显性负性Xwnt-8阻断。这些数据共同支持了一个模型，其中Wnt介导的信号对外侧神经板的图案化是建立后外侧域的早期事件，该后外侧域由Pax-3和Msx-1表达标记，随后将从该后外侧域产生Rohon-Beard细胞和神经嵴。(C)北京：人民出版社.
During early patterning of the vertebrate neuraxis, the expression of the paired-domain transcription factor Pax-3 is induced hn the lateral portions of the posterior neural plate via posteriorizing signals emanating from the late organizer and posterior nonaxial mesoderm. Using a dominant-negative approach, we show in explant assays that Pax-3 inductive activities from the organizer do not depend on FGF,retinoic acid or XWnt-8 either alone or In combination, suggesting that the organizer map produce an unknown posteriorizing factor. However, Pax-3 inductive signals from posterior nonaxial mesoderm are Wnt-dependent. We show that Pax-3 expression in the lateral neural plate expands in XWnt-8-injected embryos and is blocked by dominant-negative XWnt-8. Similarly, we show that the homeodomain transcription factor Msx-1 which like Pax-3 is an early marker of the lateral neural plate, is induced by posterior nonaxial mesoderm and blocked by dominant-negative XWnt-8. Finally, we show that Rohon-Beard primary neurons, a cell type that develops within the lateral neural plate, we also blocked in vivo by dominant-negative Xwnt-8. Together these data support a model in which patterning of the lateral neural plate by Wnt-mediated signals is an early event that establishes a posteriolateral domain, marked by Pax-3 and Msx-1 expression, from which Rohon-Beard cells and neural crest will subsequently arise. (C) 1999 Academie Press.