Confidence maps: statistical inference of cryo-EM maps.
Confidence maps: statistical inference of cryo-EM maps.
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置信图：冷冻电镜图的统计推断。
DOI：
10.1107/s2059798320002995
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Beckers M
中科院分区：
文献类型：
--
作者：
Beckers M
置信图为解释冷冻电镜密度提供补充信息，因为它们表明相对于背景噪声的统计显着性。可以通过指定预期错误发现率 (FDR) 对它们进行阈值设置，并且显示的体积显示地图中具有相应显着性级别的部分。此处回顾了置信图的基本统计概念，并为其在 CCP-EM 套件中的解释和使用提供了实用指南。讨论了该方法的局限性，并提出了对其他错误标准（例如族错误率）的扩展。观察到的地图特征可以在公共等值面阈值处渲染，这对于弱密度和噪声密度的解释特别有益。在本文中，为置信图的推荐使用提供了实用指南。
Confidence maps provide complementary information for interpreting cryo-EM densities as they indicate statistical significance with respect to background noise. They can be thresholded by specifying the expected false-discovery rate (FDR), and the displayed volume shows the parts of the map that have the corresponding level of significance. Here, the basic statistical concepts of confidence maps are reviewed and practical guidance is provided for their interpretation and usage inside the CCP-EM suite. Limitations of the approach are discussed and extensions towards other error criteria such as the family-wise error rate are presented. The observed map features can be rendered at a common isosurface threshold, which is particularly beneficial for the interpretation of weak and noisy densities. In the current article, a practical guide is provided to the recommended usage of confidence maps.