SIZE LIMIT OF MOLECULES PERMEATING JUNCTIONAL MEMBRANE CHANNELS
SIZE LIMIT OF MOLECULES PERMEATING JUNCTIONAL MEMBRANE CHANNELS
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DOI：
10.1126/science.831276
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发表时间：
1977-01-01
期刊：
影响因子：
56.9
通讯作者：
LOEWENSTEIN, WR
中科院分区：
文献类型：
--
作者：
SIMPSON, I;ROSE, B;LOEWENSTEIN, WR
用荧光标记的氨基酸和人工合成或天然的多肽，研究了唾液腺细胞交界处细胞-细胞膜通道的通透性。高达1200道尔顿的分子以取决于分子大小的速度通过通道。分子.gtoreq.1900个道尔顿不及格。这种通道故障似乎反映了连接通道渗透的正常尺寸限制；当这些分子与非预想的分子混合时，通道继续被分子渗透到1200道尔顿。根据这一尺寸限制，估计渠道直径为10-14°。
The permeability of the cell-to-cell membrane channels in salivary gland cell junction (Chironomus thummi) was probed with fluorescent-labeled amino acids and synthetic or natural peptides. Molecules up to 1200 daltons pass through the channels with velocities depending on molecular size. Molecules .gtoreq. 1900 daltons do not pass. This passage failure seems to reflect the normal size limit for junctional channel permeation; the channels continue to be permeated by the molecules up to 1200 daltons when these are mixed with the nonpermeant molecules. From this size limit a channel diameter of 10-14 .ANG. is estimated.