Gold nanorod-photosensitizer conjugates with glutathione-sensitive linkages for synergistic cancer photodynamic/photothermal therapy
Gold nanorod-photosensitizer conjugates with glutathione-sensitive linkages for synergistic cancer photodynamic/photothermal therapy
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DOI：
10.1002/bit.26536
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发表时间：
2018-05-01
影响因子：
3.8
通讯作者：
Kim, So Yeon
中科院分区：
文献类型：
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作者：
Choi, Jongseon;Lee, Sang-Eun;Kim, So Yeon
最近，光动力疗法（PDT）作为治疗各种癌症的有用方式已得到深入研究。此外，使用金纳米载体的近红外（NIR）光热疗法（PTT）作为一种热疗策略引起了特别的兴趣。在本研究中，设计了具有谷胱甘肽敏感键的金纳米棒 (AuNR)-光敏剂缀合物，用于 PDT 和 PTT。合成了几种不同长宽比的AuNRs，并用FA-共轭嵌段共聚物（FA-PEG-P(Asp)-DHLA）和二氢卟酚e6（Ce6）作为光敏剂对其进行修饰。表面修饰的 AuNRs 在水溶液中表现出优异的稳定性和溶解度。特别是长径比为3.84的FA-PEG-P(Asp)-DHLA-AuNR100-SS-Ce6表现出强烈的光热效应、增强的单线态氧生成和显着的光毒性。基于这些结果，我们认为具有谷胱甘肽敏感键的 AuNR-光敏剂缀合物在 PDT/PTT 中具有潜在的应用，可有效临床治疗各种癌症。
Recently, photodynamic therapy (PDT) has been intensively investigated as a useful modality for the treatment of various cancers. In addition, near infrared (NIR) photothermal therapy (PTT) using gold nanocarriers has attracted particular interest as a hyperthermia strategy. In this study, gold nanorod (AuNR)-photosensitizer conjugates with glutathione-sensitive linkages were designed for PDT and PTT. Several kinds of AuNRs with different aspect ratios were synthesized and modified with FA-conjugated block copolymers (FA-PEG-P(Asp)-DHLA) and Chlorin e6 (Ce6) as a photosensitizer. The surface-modified AuNRs showed excellent stability and solubility in aqueous solution. In particular, FA-PEG-P(Asp)-DHLA-AuNR100-SS-Ce6 with a 3.84 aspect ratio exhibited strong photothermal effects, enhanced singlet oxygen generation, and marked phototoxicity. Based on these results, we suggest that AuNR-photosensitizer conjugates with glutathione-sensitive linkages have potential application in PDT/PTT for effective clinical treatment of various cancers.