Monitoring insulin-stimulated production of signaling lipids at the plasma membrane.
Monitoring insulin-stimulated production of signaling lipids at the plasma membrane.
复制标题
监测质膜上胰岛素刺激的信号脂质的产生。
DOI：
10.1007/978-1-60327-378-7_4
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Frohman,MichaelA
中科院分区：
文献类型：
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作者：
Osisami,Mary;Huang,Huiyan;Frohman,MichaelA
脂质第二信使在许多细胞信号级联反应中起重要作用。脂质信号分子允许高特异性，快速转导和快速可逆性局部刺激事件。荧光传感器能够检测单个信号脂质，从而跟踪其产生和降解，揭示信号通路的性质和动力学。以下章节概述了一种使用脂质传感器监测C2C12肌管质膜上响应胰岛素信号的信号脂质的方法。
Lipid second messengers play important roles in many cell signaling cascades. Lipid signaling molecules allow for high specificity, rapid transduction, and rapid reversibility of localized stimulation events. Fluorescent sensors capable of detecting individual signaling lipids enable their production and degradation to be followed, revealing the nature and dynamics of signaling pathways. The following sections outline a method for using lipid sensors to monitor the production of signaling lipids on the plasma membrane of C2C12 myotubes in response to insulin signaling.