A mitochondria-targeted fluorescent probe for selective detection of mitochondrial labile Fe(II)
A mitochondria-targeted fluorescent probe for selective detection of mitochondrial labile Fe(II)
复制标题
DOI：
10.1039/c8mt00049b
复制
发表时间：
2018-06-01
期刊：
影响因子：
3.4
通讯作者：
Nagasawa, Hideko
中科院分区：
文献类型：
--
作者：
Hirayama, Tasuku;Kadota, Satoki;Nagasawa, Hideko
线粒体是富含铁的细胞器，通过电子传递系统和血红素合成参与能量产生过程。我们开发了一种新的线粒体靶向荧光探针MtFluNox/Ac MtFluNox，用于基于n -氧化物化学的铁(u)，我们最近将其确定为铁(u)选择性荧光开关。在试管实验中，脱乙酰化形式MtFluNox对Fe（ii）具有高金属选择性，并且使用其细胞兼容类似物Ac MtFluNox的成像研究表明，活细胞中线粒体特异性荧光增强对Fe(u)的响应。此外，探针能够检测内源性积累的铁(u)诱导的血红素合成抑制的结果。
Mitochondria are iron-rich organelles that are involved in the process of energy production through the electron-transporting system and heme synthesis. We developed a new mitochondria-targeted fluorescent probe, MtFluNox/Ac MtFluNox, for Fe(u) based on N-oxide chemistry, which we recently established as a Fe(u)-selective fluorogenic switch. The deacetyLated form MtFluNox showed a turn-on response towards Fe(ii) with high metal selectivity in cuvette experiments, and an imaging study using its cell-compatible analogue Ac MtFluNox demonstrated mitochondria-specific fluorescence enhancement in response to Fe(u) in Living cells. Furthermore, the probe was able to detect endogenously accumulated Fe(u) induced as a result of the inhibition of heme synthesis.