Berberine enhances the anti-tumor activity of tamoxifen in drug-sensitive MCF-7 and drug-resistant MCF-7/TAM cells
Berberine enhances the anti-tumor activity of tamoxifen in drug-sensitive MCF-7 and drug-resistant MCF-7/TAM cells
复制标题
小檗碱增强他莫昔芬在药物敏感 MCF-7 和耐药 MCF-7/TAM 细胞中的抗肿瘤活性
DOI：
10.3892/mmr.2016.5490
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发表时间：
2016-09-01
影响因子：
3.4
通讯作者：
Zhou, Honghao
中科院分区：
文献类型：
--
作者：
Wen, Chunjie;Wu, Lanxiang;Zhou, Honghao
黄连素是一种异喹啉生物碱，此前已被证明具有抗乳腺癌的特性。他莫昔芬被广泛用于预防和治疗雌激素受体阳性的乳腺癌。因此，本研究的目的是通过四甲基偶氮唑蓝、流式细胞仪和免疫印迹法检测黄连素是否能增强他莫昔芬的抗癌作用，并探讨其在三苯氧胺敏感和耐药细胞中的作用机制。结果表明，黄连素对MCF-7和MCF-7/细胞的增殖抑制作用呈剂量和时间依赖性。此外，黄连素和他莫昔芬联合应用对细胞生长的抑制作用比单用他莫昔芬更有效。本研究还表明，联合用药较单用他莫昔芬更有效地诱导G1期停滞和激活细胞凋亡，这可能与其上调P21表达和下调B细胞CLL/淋巴瘤2(Bcl2)/Bcl2相关X蛋白比例有关。本研究结果提示，黄连素可能在肿瘤化疗中作为一种辅助药物来增强他莫昔芬的疗效，这将有助于抗肿瘤治疗和进一步的研究。
Berberine, an isoquinoline alkaloid, has been previously demonstrated to possess anti-breast cancer properties. Tamoxifen is widely used in the prevention and treatment of estrogen receptor-positive breast cancer. Thus, the aim of the present study was to assess whether berberine enhanced the anticancer effect of tamoxifen, and the underlying mechanism involved in this combined effect in tamoxifen-sensitive (MCF-7) and tamoxifen-resistant (MCF-7/TAM) cells using MTS, flow cytometry and western blot assays. The results indicated that berberine demonstrated dose- and time-dependent anti-proliferative activity in MCF-7 and MCF-7/TAM cells. Furthermore, the combination of berberine and tamoxifen induced cell growth inhibition more effectively than tamoxifen alone. The present study also demonstrated that combinational treatment is more effective in inducing G1 phase arrest and activating apoptosis compared tamoxifen alone, which may be due to upregulation of P21 expression and downregulation of the B-cell CLL/lymphoma 2(Bcl-2)/Bcl-2 associated X protein ratio. The results of the present study suggested that berberine may potentially be useful as an adjuvant agent in cancer chemotherapy to enhance the effect of tamoxifen, which will be useful for anti-tumor therapy and further research.