HU-LI-TAI-SHAO, A GENE REQUIRED FOR RING CANAL FORMATION DURING DROSOPHILA OOGENESIS, ENCODES A HOMOLOG OF ADDUCIN
HU-LI-TAI-SHAO, A GENE REQUIRED FOR RING CANAL FORMATION DURING DROSOPHILA OOGENESIS, ENCODES A HOMOLOG OF ADDUCIN
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DOI：
10.1101/gad.6.12b.2443
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发表时间：
1992-12-01
影响因子：
10.5
通讯作者：
SPRADLING, AC
中科院分区：
文献类型：
--
作者：
YUE, L;SPRADLING, AC
雌性果蝇携带一种先前未被描述的基因突变，“哺育太少”，产生的卵室含有少于15个正常的哺育细胞，而且通常缺少一个卵母细胞。乳母细胞与卵母细胞之间的细胞质桥（环管）出现异常，缺乏相关的肌动蛋白环。发现hts位点编码哺乳动物膜骨骼蛋白内收蛋白的同源物。在卵发生过程中，hts mRNA定位于卵母细胞前部，随后在各种胚胎组织中表达。这些研究表明，果蝇内收蛋白需要在发育中的卵室细胞接触的特定区域组装肌动蛋白，并且可能在果蝇生命周期的其他时间也起作用。
Drosophila females bearing mutations in a previously undescribed gene, hu-li tai shao [(hts) too little nursing], produced egg chambers that contained fewer than the normal 15 nurse cells and that usually lacked an oocyte. The cytoplasmic bridges (ring canals) interconnecting nurse cells and the oocyte appeared abnormal, and lacked associated actin rings. The hts locus was found to encode a homolog of the mammalian membrane skeletal protein adducin. During oogenesis, hts mRNA became localized at the anterior of the oocyte and was subsequently expressed in a variety of embryonic tissues. These studies suggested that Drosophila adducin is needed to assemble actin at specialized regions of cell-cell contact in developing egg chambers and may also function at other times during the Drosophila life cycle.