EPITHELIAL MARKERS IN PRIMARY SKIN-CANCER - AN IMMUNOPEROXIDASE STUDY OF THE DISTRIBUTION OF EPITHELIAL MEMBRANE ANTIGEN (EMA) AND CARCINOEMBRYONIC ANTIGEN (CEA) IN 65 PRIMARY SKIN CARCINOMAS
EPITHELIAL MARKERS IN PRIMARY SKIN-CANCER - AN IMMUNOPEROXIDASE STUDY OF THE DISTRIBUTION OF EPITHELIAL MEMBRANE ANTIGEN (EMA) AND CARCINOEMBRYONIC ANTIGEN (CEA) IN 65 PRIMARY SKIN CARCINOMAS
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DOI：
10.1111/j.1365-2559.1984.tb02354.x
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发表时间：
1984-01-01
期刊：
影响因子：
6.4
通讯作者：
MCKEE, PH
中科院分区：
文献类型：
--
作者：
HEYDERMAN, E;GRAHAM, RM;MCKEE, PH
使用兔多克隆亲和纯化抗体和间接免疫过氧化物酶技术对原发性恶性皮肤肿瘤 (65) 进行癌胚抗原 (CEA) 和上皮膜抗原 (EMA) 染色。肿瘤包括 15 例浸润性鳞状癌、23 例基底细胞癌、16 例恶性小汗腺孔瘤（汗孔癌）和 11 例皮脂腺癌。基底细胞癌的 CEA 和 EMA 均为阴性，但存在角化或皮脂腺分化的情况除外。所有的皮脂腺癌和鳞状癌以及15/16的毛孔癌都含有EMA。 15 例鳞癌中有 12 例 CEA 呈阳性。除 2 的溃疡表面外，恶性孔瘤的 CEA 呈阴性。在分类为皮脂腺癌的肿瘤中，某些细胞、囊肿内容物和/或角化病灶中 CEA 呈阳性染色。这些发现对于使用上皮标记物的免疫过氧化物酶定位来鉴别诊断原发性和转移性皮肤癌具有重要意义。
Primary malignant skin tumors (65) were stained for carcinoembryonic antigen (CEA) and epithelial membrane antigen (EMA) using rabbit polyclonal affinity-purified antibodies and an indirect immunoperoxidase technique. The tumors consisted of 15 invasive squamous carcinomas, 23 basal cell carcinomas, 16 malignant eccrine poromas (porocarcinomas), and 11 sebaceous carcinomas. The basal cell carcinomas were negative for CEA and EMA except where there was keratotic or sebaceous differentiation. All the sebaceous and squamous carcinomas and 15/16 porocarcinomas contained EMA. Twelve of 15 squamous carcinomas were positive for CEA. The malignant poromas were negative for CEA except on the ulcerated surface of 2. In tumors classified as sebaceous carcinomas there was positive staining for CEA in some cells, cyst contents and/or keratotic foci. These findings have implications for the use of immunoperoxidase localization of epithelial markers in the differential diagnosis of primary and metastatic skin cancer.