A sensitive gonadotropin responsive system: radioimmunoassay of testosterone production by the rat testis in vitro.
A sensitive gonadotropin responsive system: radioimmunoassay of testosterone production by the rat testis in vitro.
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敏感的促性腺激素反应系统：体外大鼠睾丸产生睾酮的放射免疫测定。
DOI：
10.1210/endo-90-4-1032
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发表时间：
1972
期刊：
影响因子：
4.8
通讯作者：
T. Tsuruhara
中科院分区：
文献类型：
--
作者：
M. Dufau;K. Catt;T. Tsuruhara
用放射免疫法测定体外培养的大鼠睾丸对促性腺激素释放的睾酮与人绒毛膜促性腺激素(HCG)和黄体生成素(LH)的促性腺激素反应。在用竞争性蛋白结合法和放射免疫法测定之前，参照提取和层析的特异性睾酮测定，验证了用孵育液的放射免疫法测定睾酮的方法。在单个睾丸的体外孵育过程中，睾酮的产生缓慢上升到基础水平，即100-200 ng/睾丸/4小时。加入20 ng(7.2×10~(-11)M)的HCG或10~(-3)M的二丁酰环AMP可显著增加睾酮的产生，在注射后1min即可检测到，并在整个孵育期内增加。体外分离的成年大鼠睾丸对促性腺激素刺激极为敏感，低至1mLU(0.2 ng)hCG、2.5mLU(0.7 ng)人促黄体生成素(HLH)、0 mLU(0.2 ng)人促黄体生成素(HCG)、0 mLU(0.2 ng)hCG、0 mLU(0.7 ng)hCG、0 mLU(0.2 ng)hCG、0 mLU(0.7 ng)hCG、0 mLU(0.2 ng)hCG、0 mLU(0.7 ng)人促黄体生成素均能刺激睾丸释放睾酮.
The steroidogenic response of the isolated rat testis to gonadotropic hormones was measured by radioimmunoassay of testosterone released during incubation in vitro with human chorionic gonadotropin (HCG) and luteinizing hormone (LH). Measurement of testosterone production by radioimmunoassay of incubation media was validated by reference to specific testosterone assays employing extraction and chromatography prior to measurement by competitive protein binding and radioimmunoassay. During incubation of individual testes in vitro, testosterone production rose slowly to the basal level of 100-200 ng/testis/4 hr. Addition of 20 ng (7.2 X 10-11M) HCG or 10-3M dibutyryl cyclic AMP caused a marked rise in testosterone production, detectable after IS min and increasing throughout the period of incubation. The decapsulated adult rat testis was extremely sensitive to gonadotropin stimulation in vitro, responding with increased testosterone release to as little as 1 mlU (0.2 ng) HCG, 2.5 mlU (0.7 ng) human LH, and 0...