The effects of low-color-temperature dual-primary-color light-emitting diodes on three kinds of retinal cells
The effects of low-color-temperature dual-primary-color light-emitting diodes on three kinds of retinal cells
复制标题
低色温双基色发光二极管对三种视网膜细胞的影响
DOI：
10.1016/j.jphotobiol.2020.112099
复制
发表时间：
2021-01-01
影响因子：
5.4
通讯作者：
Zhang, Xu
中科院分区：
文献类型：
--
作者：
Jin, Ming;Li, Xiongfeng;Zhang, Xu
用蓝光激发的荧光粉转换发光二极管(LED)长期照射视网膜可能会导致视网膜功能下降，即使发出的蓝光水平很低。新型低色温双基色LED已经被开发出来，它只由两个LED芯片组成：一个红色芯片和一个黄色芯片。这些LED不发射蓝光，缺乏荧光粉，解决了荧光粉转换的低色温LED效率低的问题，有望成为一种新型的健康照明光源。许多研究表明，这些新的低色温LED很可能具有治疗作用。然而，在探索治疗效果之前，需要探索这些LED的生物安全性。因此，本实验研究了新型低色温LED和荧光白光LED对三种类型视网膜细胞的影响。我们观察到，随着LED色温的升高，视网膜细胞的活力和数量逐渐减少。与荧光白色LED相比，新的低色温LED导致的死亡和对增殖的不良影响更少。高色温LED照射后，ARPE-19细胞ZO-1表达减少且不连续，RMC-1细胞HO-1表达上调，胶质纤维酸性蛋白(GFAP)和波形蛋白表达上调。因此，我们得出结论，新的白色LED几乎不会对视网膜细胞造成损害，并降低了长期暴露在荧光白色LED下的潜在人类健康风险。
Long-term illumination of the retina with blue-light-excited phosphor-converted light-emitting diodes (LEDs) may result in decreased retinal function, even if the levels of blue light emitted are low. New low-color temperature dual-primary-color LEDs have been developed that are composed of only two LED chips: a red chip and a yellow chip. These LEDs are expected to become a new type of healthy lighting source because they do not emit blue light, they lack phosphor, and they solve the problem of low efficiency encountered with phosphor converted low-color-temperature LEDs. Many studies have indicated that these new low-color-temperature LEDs are likely to have therapeutic effects. However, the biological safety of these LEDs needs to be explored before the therapeutic effects are explored. Therefore, this experiment was conducted to investigate the effects of the new low-color-temperature LEDs and fluorescent white LEDs on three types of retinal cells. We observed that the viability and numbers of retinal cells decreased gradually with increasing LED color temperature. The new low color-temperature LEDs caused less death and adverse effects on proliferation than the fluorescent white LEDs. After irradiation with high-color-temperature LEDs, the expression of Zonula Occludens-1 (ZO-1) was decreased and discontinuous in ARPE-19 cells; the stress protein hemeoxygenase-1 (HO-1) was upregulated in R28 cells; and glial fibrillary acidic protein (GFAP) and vimentin were upregulated in rMC-1 cells. We therefore conclude that the new white LEDs cause almost no damage to retinal cells and reduce the potential human health risks of chronic exposure to fluorescent white LEDs.