Imaging receptor microdomains on leukocyte subsets in live mice
Imaging receptor microdomains on leukocyte subsets in live mice
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DOI：
10.1038/nmeth1018
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发表时间：
2007-03-01
期刊：
影响因子：
48
通讯作者：
Frenette, Paul S.
中科院分区：
文献类型：
--
作者：
Chiang, Elaine Y.;Hidalgo, Andres;Frenette, Paul S.
我们提出了一种简单的方法来确定白细胞亚群的募集，并确定活体小鼠的并发表面受体聚集。我们发现CD45(+)F4/80-Gr-1(+)中性粒细胞在手术激活的分泌静脉中被广泛招募，而贴壁的CD45(+)B220(+)B淋巴细胞在骨髓小静脉中占主导地位。大多数贴壁的Gr-1(+)白细胞不是稳定静止的，而是活跃地迁移到肿瘤坏死因子-α激活的培养的小静脉内皮细胞上，并表现出表面PSGL-1的明显极化，而不是LFA-1，到尾缘。
We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45(+) F4/80-Gr-1(+) neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45(+) B220(+) B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1(+) leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.