Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis
Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis
复制标题
益生菌与需氧成分的混合物促进了 DSS 诱导的慢性结肠炎的多重屏障的恢复。
DOI：
10.1016/j.lfs.2019.117089
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发表时间：
2020-01-01
期刊：
影响因子：
6.1
通讯作者：
Hou, Xiaohua
中科院分区：
文献类型：
--
作者：
Chen, Yuhua;Zhang, Lei;Hou, Xiaohua
目的：肠道微生物区系与粘膜免疫密切相关，被认为是肠道炎症的可靠靶点。本研究旨在探讨婴儿双歧杆菌、嗜酸乳杆菌、粪肠球菌与(四重益生菌，P-quA)或不(三重益生菌，P-tri)需氧蜡状芽孢杆菌的混合益生菌在结肠炎中的治疗作用，重点探讨其多重屏障作用。材料和方法：用葡聚糖硫酸钠(DSS)诱导C57BL/6小鼠慢性结肠炎。分别给予益生菌混合物P-quA或P-tri，同时以粪便微生物区系移植(FMT)为阳性对照。评估肠道炎症和多种屏障的功能，包括粘液屏障、上皮屏障和称为肠血管屏障(GVB)的内皮屏障。通过测序分析观察肠道微生物区系组成和多样性的变化。主要发现：P-QuA和P-tri都减轻了DSS-结肠炎的肠道炎症，改善了多种屏障的功能，增加了粘液层的完整性，增强了跨上皮电阻，降低了上皮和内皮对大分子的通透性。与P-tri相比，含Aerobe的P-qua具有更积极的屏障修复作用，而作为阳性对照的FMT似乎比纯益生菌获得了更好的效果。的确，P-QUA能有效地重建DSS-结肠炎患者的肠道菌群结构和多样性，尤其是双歧杆菌、阿克曼杆菌、乳杆菌和类杆菌的丰度增加。意义：含氧的P-QUA通过恢复肠道微生物群和恢复炎症肠道的多屏障，是治疗慢性结肠炎的有效辅助疗法。
Aims: Gut microbiota has been closely linked to the mucosal immune and been regarded as a reliable target for intestinal inflammation. This study aimed to explore the therapeutic roles of probiotic mixtures of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis with (quadruple probiotics, P-qua) or without (triple probiotics, P-tri) aerobic Bacillus cereus in colitis, focusing on the multiple barrier functions.Materials and methods: Chronic colitis was induced by dextran sulfate sodium (DSS) in C57BL/6 mice. The probiotic mixtures P-qua or P-tri was gavage administrated respectively, while fecal microbiota transplantation (FMT) as a positive control. The intestinal inflammation and functions of multiple barriers were assessed, including the mucus barrier, epithelial barrier and endothelial barrier known as gut-vascular barrier (GVB). Altered composition and diversity in gut microbiota were observed via sequencing analysis.Key findings: Both P-qua and P-tri relieved the intestinal inflammation and improved the functions of multiple barriers with increased integrity of mucous layer, enhanced transepithelial electrical resistance, declined epithelial and endothelial permeability to macromolecules in DSS-colitis. Aerobe-contained P-qua revealed a more active role in barrier recovering relative to P-tri, while FMT as a positive control seemed to get better results than pure probiotics. Indeed, P-qua was effective in rebuilding the structure and diversity of gut flora in DSS-colitis, especially increased abundance of Bifidobacterium, Akkermansia, Lactobacillus and Bacteroides.Significance: Aerobe-contained P-qua was a powerful adjuvant therapy for chronic colitis, via restoring the intestinal microflora and recovering the multi-barriers in the inflamed gut.