Repeated methamphetamine treatment increases spine density in the nucleus accumbens of serotonin transporter knockout mice
Repeated methamphetamine treatment increases spine density in the nucleus accumbens of serotonin transporter knockout mice
复制标题
DOI：
10.1002/npr2.12049
复制
发表时间：
2019-06-01
影响因子：
2.5
通讯作者：
Sora, Ichiro
中科院分区：
文献类型：
--
作者：
Kasahara, Yoshiyuki;Sakakibara, Yasufumi;Sora, Ichiro
目的：啮齿动物反复接受精神兴奋药物（包括甲基苯丙胺）治疗很容易产生行为过敏，这反映了反复接触药物引起的大脑功能改变。伏隔核中中型多棘神经元的树突重塑被认为是行为敏化的重要机制。我们最近表明，长期甲基苯丙胺治疗不会对血清素转运蛋白敲除小鼠产生行为致敏。方法：在本研究中，我们报告了重复注射甲基苯丙胺后伏隔核中中型多棘神经元的棘密度，以检查血清素转运蛋白敲除小鼠的形态变化。结果：高尔基-COX染色清楚地表明，中型多棘神经元的棘密度 在野生型和血清素转运蛋白敲除小鼠中反复进行甲基苯丙胺治疗后，伏核中的多刺神经元增加。结论：我们的结果表明，血清素转运蛋白缺失产生的血清素能神经传递增强，以一种独立于伏核树突重塑的方式阻止了行为敏化的发展。
Aim: Repeated psychostimulant drug treatment, including methamphetamine, in rodents readily produces behavioral sensitization, which reflects altered brain function caused by repeated drug exposure. Dendritic remodeling of medium spiny neurons in the nucleus accumbens is thought to be an essential mechanism underlying behavioral sensitization. We recently showed that chronic methamphetamine treatment did not produce behavioral sensitization in serotonin transporter knockout mice.Methods: In this study, we report the spine density of medium spiny neurons in the nucleus accumbens after repeated methamphetamine injection to examine morphological alterations in serotonin transporter knockout mice.Results: Golgi-COX staining clearly showed that the spine density of medium spiny neurons in the nucleus accumbens increased following repeated methamphetamine treatment in both wild-type and serotonin transporter knockout mice.Conclusions: Our results suggested that augmented serotonergic neurotransmission produced by serotonin transporter deletion prevents the development of behavioral sensitization in a manner that is independent of dendritic remodeling in the nucleus accumbens.