High-level expression of the Japanese encephalitis virus E protein by recombinant vaccinia virus and enhancement of its extracellular release by the NS3 gene product.
High-level expression of the Japanese encephalitis virus E protein by recombinant vaccinia virus and enhancement of its extracellular release by the NS3 gene product.
复制标题
重组牛痘病毒高水平表达日本脑炎病毒E蛋白，并通过NS3基因产物增强其细胞外释放。
作者：
T. Sato;C. Takamura;A. Yasuda;M. Miyamoto;K. Kamogawa;K. Yasui
利用人工合成的启动子构建了表达日本脑炎病毒（JEV）prM和E基因的重组痘苗病毒。虽然具有优化的牛痘晚期基因启动子的重组病毒mOJ 6-SL在感染的细胞中产生了20倍升高水平的E蛋白以及86-kDa的前体蛋白，但在由mOJ 6-SL产生的细胞外或细胞表面E蛋白与由具有7.5-kDa启动子的mOJ 6产生的E蛋白之间没有检测到显著的定量差异。然而，当细胞在用mOJ 6-SL感染之前用登革2型病毒感染时，细胞外E蛋白的量增加了16倍。此外，当用表达JEV的NS 3基因的重组痘苗病毒和mOJ 6-SL共感染细胞时，观察到其胞外释放的增强。
Recombinant vaccinia viruses expressing the prM and E genes of the Japanese encephalitis virus (JEV) were constructed by use of synthetic promoters. While the recombinant virus mOJ6-SL, with an optimized vaccinia late-gene promoter, produced a 20-fold elevated level of E protein, as well as an 86-kDa precursor protein in infected cells, no significant quantitative difference was detected between the extracellular or cell-surface E protein produced by mOJ6-SL and those produced by mOJ6 with the 7.5-kDa promoter. However, when the cells were infected with Dengue 2 virus before infection with mOJ6-SL, the amount of the extracellular E protein increased 16-fold. In addition, enhancement of its extracellular release was observed when cells were co-infected with mOJ6-SL and recombinant vaccinia virus expressing the NS3 gene of JEV.