Effect of scavengers in acidolytic cleavage of Cys(Acm)-containing peptides from solid support: Isolation of an ethanedithiol disulfide adduct.
Effect of scavengers in acidolytic cleavage of Cys(Acm)-containing peptides from solid support: Isolation of an ethanedithiol disulfide adduct.
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DOI：
10.1016/0040-4039(96)00614-4
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发表时间：
1996-06-10
影响因子：
1.8
通讯作者：
Williams, NE
中科院分区：
文献类型：
--
作者：
Singh, PR;Rajopadhye, M;Williams, NE
从Wang （HMP）树脂中酸解裂解含c端Cys（Acm）的三肽可导致Acm基团的部分去保护和随后的二硫形成。发现水的存在和鸡尾酒混合物中使用的清除剂类型对这些杂质形成的程度起作用。(C) 1996杜邦默克制药公司
Acidolytic cleavage of C-terminal Cys(Acm)-containing tripeptides from the Wang (HMP) resin can lead to partial deprotection of the Acm group and subsequent disulfide formation. The presence of water and the type of scavenger used in the cocktail mixture were found to play a role in the extent of these impurities formed. (C) 1996 The DuPont Merck Pharmaceutical Company