Lactoferrin, transferrin and acidic isoferritins: regulatory molecules with potential therapeutic value in leukemia.
Lactoferrin, transferrin and acidic isoferritins: regulatory molecules with potential therapeutic value in leukemia.
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乳铁蛋白、转铁蛋白和酸性异铁蛋白：在白血病中具有潜在治疗价值的调节分子。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
P. Ralph
中科院分区：
文献类型：
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作者：
H. Broxmeyer;P. Gentile;J. Bognacki;P. Ralph
在评价乳铁蛋白、转铁蛋白和酸性异铁蛋白的纯化制剂在骨髓生成调节中的作用的过程中，发现：（1）在正常供体的血清和血浆中报道的乳铁蛋白值在大多数情况下是高估的，（2）乳铁蛋白抑制在T细胞缺乏的情况下粒细胞-巨噬细胞集落刺激因子（GM-CSF）从单核细胞的产生/释放。淋巴细胞，并且还抑制单核细胞产生/释放酸性异铁蛋白抑制活性，（3）乳铁蛋白、转铁蛋白和酸性异铁蛋白作用于它们表达Ia样抗原的特异性靶细胞，（4）乳铁蛋白和转铁蛋白在体内抑制从亚致死剂量的环磷酰胺恢复的小鼠中的反弹性骨髓生成，初步观察表明乳铁蛋白对骨髓具有更明显的作用，而转铁蛋白对脾具有更明显的作用，（5）活性乳铁蛋白来源于CML患者以及正常供体的PMN的Fc受体阳性亚群，但CML中含有Fc受体的PMN的百分比较低，在其PMN中发现的活性乳铁蛋白的量也较低，和（6）乳铁蛋白、转铁蛋白和酸性异铁蛋白抑制U937克隆形成细胞殖民地形成，其中乳铁蛋白和转铁蛋白减少刺激U937集落形成所需的生长因子从U937细胞的释放，并且乳铁蛋白和酸性异铁蛋白抑制WEHI-3细胞殖民地形成，其中乳铁蛋白减少刺激WEHI-3集落形成所需的生长因子从WEHI-3细胞的释放。推测这些铁结合糖蛋白的潜在用途，以控制疾病进展的讨论。
In the process of evaluating roles for purified preparations of lactoferrin, transferrin and acidic isoferritins in the regulation of myelopoiesis, it was found that: (1) values reported for lactoferrin in the serum and plasma of normal donors are in most cases an over-estimation, (2) lactoferrin suppresses the production/release of granulocyte-macrophage colony stimulatory factors (GM-CSF) from monocytes in the absence of T-lymphocytes and also suppresses the production/release of acidic isoferritin-inhibitory activity from monocytes, (3) lactoferrin, transferrin and acidic isoferritins act on their specific target cells which express Ia-like antigens, (4) lactoferrin and transferrin act in vivo to suppress rebound myelopoiesis in mice recovering from sublethal dosages of Cytoxan, with preliminary observations suggesting that lactoferrin has a greater apparent effect on the bone marrow and transferrin has a greater apparent effect on the spleen, (5) active lactoferrin derives from Fc receptor positive subpopulations of PMN from patients with CML as well as from normal donors, but the percentage of Fc receptor containing PMN is lower in CML, as is the amount of active lactoferrin found in their PMN, and (6) lactoferrin, transferrin and acidic isoferritins suppress the colony formation of U937 clonogenic cells, with lactoferrin and transferrin decreasing the release of growth factors from U937 cells which are needed to stimulate U937 colony formation, and lactoferrin and acidic isoferritins suppress the colony formation of WEHI-3 cells, with lactoferrin decreasing the release of growth factors from WEHI-3 cells which are needed to stimulate WEHI-3 colony formation. Speculation on the potential usefulness of these iron binding glycoproteins to control of disease progression is given in the discussion.