Template-directed reversible photocircularization of DNA via 5-vinyldeoxycytidine
Template-directed reversible photocircularization of DNA via 5-vinyldeoxycytidine
复制标题
DOI：
10.1016/s0040-4039(00)01025-x
复制
发表时间：
2000-08-12
影响因子：
1.8
通讯作者：
Saito, I
中科院分区：
文献类型：
--
作者：
Fujimoto, K;Matsuda, S;Saito, I
用5-乙烯基脱氧胞苷（C-V）对寡脱氧核苷酸（ODNs）进行了高效、可逆的模板定向光定位。在模板ODN存在的情况下，将5‘端含有C-V的ODN与3’端含有胸腺嘧啶的ODN进行光照，观察到有效的光化作用。证明了利用C-V进行光环化的环状DNA的可逆光锁。(C) 2000 Elsevier Science Ltd.版权所有。
An efficient and reversible template-directed photoligation of oligodeoxynucleotides (ODNs) using 5-vinyldeoxycytidine (C-V) was described. When ODN containing C-V at the 5' end was photoirradiated with ODN containing thymine at the 3' end in the presence of a template ODN, an efficient photoligation was observed. Reversible photopadlocking of a circular DNA by the photocircularization using C-V was demonstrated. (C) 2000 Elsevier Science Ltd. All rights reserved.