A sandwich-configuration electrochemiluminescence immunoassay based on Cu2O@OMC-Ru nanocrystals and OMC-MoS2 nanocomposites for determination of alpha-fetoprotein
A sandwich-configuration electrochemiluminescence immunoassay based on Cu2O@OMC-Ru nanocrystals and OMC-MoS2 nanocomposites for determination of alpha-fetoprotein
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基于 Cu2O@OMC-Ru 纳米晶体和 OMC-MoS2 纳米复合材料的夹心结构电化学发光免疫分析法测定甲胎蛋白
DOI：
10.1007/s00604-021-04848-4
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发表时间：
2021-05
影响因子：
5.7
通讯作者：
Chang Dong
中科院分区：
文献类型：
--
作者：
Zhang Ze;Yu Hongwei;Zhang Yingcong;Wang Zhangmin;Gao Hongmin;Rong Shengzhong;Meng Lingqiang;Dai Jianmin;Pan Hongzhi;Chang Dong
A sandwich-format electrochemiluminescence (ECL) immunosensor has been developed for alpha-fetoprotein (AFP) detection based on the use of ordered mesoporous carbon-molybdenum disulfide (OMC-MoS2) as a sensor platform and cuprous oxide @ ordered mesoporous carbon-Ru(bpy)32+(Cu2O@OMC-Ru) composites as signal tags. OMC alongside MoS2plays a synergistic role in improving the electrochemical performance of the electrode in the electron transfer process. The uniform cubic-shaped Cu2O@OMC-Ru nanocrystals display excellent luminous efficiency, with a signal amplification strategy of OMC-MoS2synergistic enhancement and Cu2O@OMC which is capable of immobilizing more Ru(bpy)32+serving as a tracing tag to label antibodies. A detectable ECL emission at a Cu2O@OMC-Ru nanocrystals modified electrode is initiated at an applied voltage of +1.15 V (scanning range: 0-1.2 V), in the presence of the tripropylamine (TPA) as coreactant. With the increase in AFP concentration, the loading of Cu2O@OMC-Ru at the electrode increases. Afterward, the ECL detection of AFP shows a wide linear range from 0.1 pg/mL to 10 ng/mL with a correlation coefficient of 0.9964 and a detection limit of 0.011 pg/mL (S/N = 3) under the optimal experimental conditions. The recoveries were in the range 91.2-97.1% with RSD varying from 4.8 to 8.5%. Overall, the novel immunosensor has been successfully applied to the analysis of human serum samples, indicating a great potential for application in clinical diagnostics.
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