Efficient colonization of the endophytes Herbaspirillum huttiense RCA24 and Enterobacter cloacae RCA25 influences the physiological parameters of Oryza sativa L. cv. Baldo rice
Efficient colonization of the endophytes Herbaspirillum huttiense RCA24 and Enterobacter cloacae RCA25 influences the physiological parameters of Oryza sativa L. cv. Baldo rice
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DOI：
10.1111/1462-2920.14688
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发表时间：
2019-09-01
影响因子：
5.1
通讯作者：
Bianco, Carmen
中科院分区：
文献类型：
--
作者：
Andreozzi, Anna;Prieto, Pilar;Bianco, Carmen
一些重要的细菌特性，如生物固氮、磷酸盐溶解、1-氨基环丙烷-1-羧酸脱氨酶活性、铁载体和植物激素的产生，可以被评估为植物生长促进性状。本研究旨在评价两个水稻品种（Baldo和Vialone Nano）固氮和内生菌产生吲哚-3-乙酸（IAA）的效果。研究了胡氏Herbaspirillum hutiense RCA24、asburiae Enterobacter asburiae RCA23和Staphylococcus sp. 377三种产生不同IAA水平的细菌对阴沟肠杆菌RCA25中niifh基因表达和氮酶活性的影响。结果表明，胡家槐RCA24表现最佳。与菌株RCA25和RCA24按10:1的比例共接种后，植株的固氮性得到改善，叶绿素、氮含量和茎部干重等生理参数也发生了变化。共聚焦激光扫描显微镜分析结果表明，菌株RCA24是根内最佳定殖菌，也是唯一与RCA25细胞占据同一根生态位的IAA产生菌。这项工作表明，选择具有正确组成的生物接种剂是接种具有微生物联合体的特定寄主植物品种需要考虑的关键方面之一。
Several important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, 1-aminocyclopropane-1-carboxylate deaminase activity and production of siderophores and phytohormones, can be assessed as plant growth promotion traits. Our aim was to evaluate the effects of nitrogen fixing and indole-3-acetic acid (IAA) producing endophytes in two Oryza sativa cultivars (Baldo and Vialone Nano). Three bacteria, Herbaspirillum huttiense RCA24, Enterobacter asburiae RCA23 and Staphylococcus sp. 377, producing different IAA levels, were tested for their ability to enhance nifH gene expression and nitrogenase activity in Enterobacter cloacae RCA25. Results showed that H. huttiense RCA24 performed best. Improvement in nitrogen fixation and changes in physiological parameters such as chlorophyll, nitrogen content and shoot dry weight were observed for plants co-inoculated with strains RCA25 and RCA24 in a 10:1 ratio. Based on confocal laser scanning microscopy analysis, strain RCA24 was the best colonizer of the root interior and the only IAA producer located in the same root niche occupied by RCA25 cells. This work shows that the choice of a bio-inoculum having the right composition is one of the key aspects to be considered for the inoculation of a specific host plant cultivar with microbial consortia.