A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis.
A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis.
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拟南芥中的 GPAT1 突变提高了株高，但损害了种子油的生物合成
DOI：
10.3390/ijms22020785
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发表时间：
2021-01-14
影响因子：
5.6
通讯作者：
Liang C
中科院分区：
文献类型：
--
作者：
Bai Y;Shen Y;Zhang Z;Jia Q;Xu M;Zhang T;Fang H;Yu X;Li L;Liu D;Qi X;Chen Z;Wu S;Zhang Q;Liang C
甘油-3-磷酸酰基转移酶(GPATs)在甘油脂生物合成中起重要作用，主要参与油脂生产、花发育和逆境反应。然而，它们在调节植株高度中的作用仍然没有报道。在此，我们报道了拟南芥GPAT1参与了株高的调节。GUS分析和qRT-PCR分析表明，GPAT1在花、角果和种子中高表达。GPAT1功能缺失突变可通过增加细胞长度来降低种子产量，增加株高。转录和qRT-PCR结果表明，与赤霉素(GA)生物合成和信号转导相关的基因以及细胞壁组织和生物发生相关基因的表达水平显著上调。这导致了细胞长度的延长，从而增加了植株的高度。综上所述，我们的数据表明，GPAT1基因的敲除损害了拟南芥的甘油脂代谢，导致种子产量下降，但促进了GA的生物合成，最终提高了植株高度。这项研究为脂类和激素代谢在株高调节中的相互作用提供了新的证据。
Glycerol-3-phosphate acyltransferases (GPATs) play an important role in glycerolipid biosynthesis, and are mainly involved in oil production, flower development, and stress response. However, their roles in regulating plant height remain unreported. Here, we report that Arabidopsis GPAT1 is involved in the regulation of plant height. GUS assay and qRT-PCR analysis in Arabidopsis showed that GPAT1 is highly expressed in flowers, siliques, and seeds. A loss of function mutation in GPAT1 was shown to decrease seed yield but increase plant height through enhanced cell length. Transcriptomic and qRT-PCR data revealed that the expression levels of genes related to gibberellin (GA) biosynthesis and signaling, as well as those of cell wall organization and biogenesis, were significantly upregulated. These led to cell length elongation, and thus, an increase in plant height. Together, our data suggest that knockout of GPAT1 impairs glycerolipid metabolism in Arabidopsis, leading to reduced seed yield, but promotes the biosynthesis of GA, which ultimately enhances plant height. This study provides new evidence on the interplay between lipid and hormone metabolism in the regulation of plant height.
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