Serum-free medium for the in vitro growth of normal and malignant urinary bladder epithelial cells.
Serum-free medium for the in vitro growth of normal and malignant urinary bladder epithelial cells.
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用于正常和恶性膀胱上皮细胞体外生长的无血清培养基。
作者：
E. Messing;J. Fahey;J. Dekernion;S. Bhuta;J. E. Bubbers
本文介绍了一种无血清培养液--DH-S1，它对建立正常和恶性移行上皮(膀胱移行细胞癌)的原代培养很有价值。促上皮细胞生长但抑制成纤维细胞生长。另一种已建立的人移行细胞癌细胞系647V已在DH-S1中连续生长了36代以上。对647V细胞在含血清和无血清培养基中生长的形态和抗原性研究表明，在低密度时差异明显，但在高密度时几乎消失。描述了一种新的人移行细胞癌细胞系LA-B1，它的初始生长是由这种无血清培养基所支持的。
A serum-free medium, DH-S1, is described which is valuable for the establishment of primary cultures of normal and malignant transitional epithelium (transitional cell carcinoma of the bladder). Growth of epithelial cells in DH-S1 is facilitated but that of fibroblasts is suppressed. Another established human transitional cell carcinoma cell line, 647V, has grown continually in DH-S1 for over 36 passages. Morphological and antigenic studies comparing 647V cells growing in serum-containing and serum-free medium reveal differences which are pronounced at low cell density but which almost disappear at higher densities. A new human transitional cell carcinoma cell line, LA-B1, is described whose initial growth was supported by this serum-free medium.