The genome of the rice planthopper egg parasitoid wasps Anagrus nilaparvatae casts light on the chemo- and mechanosensation in parasitism.
The genome of the rice planthopper egg parasitoid wasps Anagrus nilaparvatae casts light on the chemo- and mechanosensation in parasitism.
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DOI：
10.1186/s12864-022-08656-9
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发表时间：
2022-07-28
期刊：
影响因子：
4.4
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中科院分区：
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小蜂科（Mymaridae）是一个古老的昆虫类群，是小蜂总科（Chalcidoidea）的一个基本谱系。小蜂科昆虫在生物防治方面具有很大的潜力。稻虱蚜小蜂是稻飞虱科的代表性昆虫，寄生率高，在田埂上能迅速找到寄主，是防治稻飞虱的理想害虫。我们使用PacBio单分子实时和Illumina测序，对A.这是蚁科的首次发现。该组件由394个支架组成，总计488.8 Mb。组装体具有高度的连续性和完整性，基准通用单拷贝骨科的N50值为25.4 Mb，映射率为98.2%。总共注释了基因组中的16，894个蛋白质编码基因。构建了A. nilaparvatrium和其他12种Hypltera证实Mymaridae是所有剩余小蜂的姐妹。A. nilaparvatrium和其他7种小蜂总科昆虫的年代约为126.9 Mya。化学感受器和机械感受器基因在解释寄生行为中是重要的。我们在A. nov.的基因组中鉴定了17种气味结合蛋白、11种化学感受蛋白、4种Niemann-Pick C2型蛋白、88种嗅觉受体、12种味觉受体、22种离子型受体和13种感觉神经元膜蛋白。与化学感受功能相关的Nilaparvastine。引人注目的是，基因组中只有一个扒手受体和九个瞬时受体潜力基因具有机械感觉功能。我们获得了高质量的A.利用PacBio单分子实时测序技术对尼拉帕韦进行测序，这为尼拉帕韦的进化历史提供了基因组学方面的见解。在A. Nilaparventrium的种特异性寄主检测和产卵行为。尼拉伐他汀可能通过相对简单的分子途径调节。在线版本包含补充材料，可通过10.1186/s12864-022-08656-9获得。
Mymaridae is an ancient insect group and is a basal lineage of the superfamily Chalcidoidea. Species of Mymaridae have great potential for biological control. Anagrus nilaparvatae, a representative species of Mymaridae, is ideal for controlling rice planthopper due to its high rate of parasitism and ability to find hosts efficiently in paddy ridges and fields. Using both PacBio single-molecule real-time and Illumina sequencing, we sequenced and assembled the whole genome of A. nilaparvatae, a first for the family Mymaridae. The assembly consists of 394 scaffolds, totaling 488.8 Mb. The assembly is of high continuity and completeness, indicated by the N50 value of 25.4 Mb and 98.2% mapping rate of Benchmarking Universal Single-Copy Orthologs. In total, 16,894 protein-coding genes in the genome were annotated. A phylogenomic tree constructed for A. nilaparvatae and other 12 species of Hymenoptera confirmed that the family Mymaridae is sister to all remaining chalcidoids. The divergence time between A. nilaparvatae and the other seven Chalcidoidea species was dated at ~ 126.9 Mya. Chemoreceptor and mechanoreceptor genes are important in explaining parasitic behavior. We identified 17 odorant binding proteins, 11 chemosensory proteins, four Niemann-Pick type C2 proteins, 88 olfactory receptors, 12 gustatory receptors, 22 ionotropic receptors and 13 sensory neuron membrane proteins in the genome of A. nilaparvatae, which are associated with the chemosensory functions. Strikingly, there is only one pickpocket receptors and nine transient receptor potential genes in the genome that have a mechanosensory function. We obtained a high-quality genome assembly for A. nilaparvatae using PacBio single-molecule real-time sequencing, which provides phylogenomic insights for its evolutionary history. The small numbers of chemo- and mechanosensory genes in A. nilaparvatae indicate the species-specific host detection and oviposition behavior of A. nilaparvatae might be regulated by relatively simple molecular pathways. The online version contains supplementary material available at 10.1186/s12864-022-08656-9.
影响因子：
3.7
作者：
Labaj PP;Linggi BE;Wiley HS;Kreil DP
通讯作者：
Kreil DP