Histological and neurotrophic changes triggered by varying models of bladder inflammation
Histological and neurotrophic changes triggered by varying models of bladder inflammation
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DOI：
10.1016/s0022-5347(05)65931-9
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发表时间：
2001-09-01
影响因子：
6.6
通讯作者：
Steers, WD
中科院分区：
文献类型：
--
作者：
Dupont, MC;Spitsbergen, JM;Steers, WD
目的：我们确定膀胱炎是否导致膀胱实质细胞神经生长因子的表达增加，从而导致支配膀胱的神经元的改变。为了回答这一问题，在建立了不同的膀胱炎模型后，获得了大鼠的生化、组织学和神经元大小的数据。材料和方法：化学(2.5%福尔马林)，免疫(脂多糖2×10(4)cfu/ml。)在不同时间对机械性(铬线)炎症进行评估，并与对照组膀胱进行比较。苏木精-伊红染色和Giemsa染色用于表征炎症和量化膀胱内的肥大细胞。采用双酶联免疫吸附试验和逆转录聚合酶链式反应分别检测大鼠膀胱和盆神经节组织中神经生长因子蛋白和信使RNA的含量。结果：各种炎症均可使大鼠膀胱重量增加，并出现弥漫性增生、壁内水肿、急、慢性炎症细胞、细胞浸润和肥大细胞增多。一般情况下，膀胱炎导致神经生长因子升高50%，周围神经元增大52%~58%。结论：炎症导致膀胱神经生长因子含量改变，支配膀胱的感觉神经元和运动神经元发生形态变化。这种神经可塑性可能是膀胱炎与炎症消退后的长期症状和疼痛相关的一个可能的解释。
Purpose: We determined whether bladder inflammation causes elevated expression of nerve growth factor by bladder parenchymal cells, leading to alterations in neurons innervating the bladder. To answer this question biochemical, histological and neuronal size data were obtained in rats following various experimental models of bladder inflammation.Materials and Methods: Chemical (2.5% formalin), immune (lipopolysaccharide 2 x 10(4) cfu/ml.) and mechanical (chromic catgut) inflammation was evaluated at various times and compared to control bladders. Hematoxylin and eosin, and Giemsa staining was done to characterize inflammation and quantify mast cells in the bladder. Nerve growth factor protein and messenger RNA were assayed in the bladder and major pelvic ganglion using 2-site enzyme-linked immunosorbent assay and reverse transcriptase-polymerase chain reaction, respectively. Retrograde axonal tracing was done to size bladder neurons in the major pelvic and dorsal root ganglia.Results: All forms of inflammation increased bladder weight and produced diffuse hyperplasia, intramural edema, acute and chronic inflammatory cells, infiltration and mastocytosis. Generally bladder inflammation resulted in a 50% increase in nerve growth factor and 52% to 58% enlargement of peripheral neurons.Conclusions: Inflammation results in altered nerve growth factor content of the bladder, and morphological changes in sensory and motor neurons innervating the bladder. Such neuroplasticity may be a possible explanation for the association of bladder inflammation with long-term symptoms and pain after inflammation subsides.