Spiroplasma citri movement into the intestines and salivary glands of its leafhopper vector, Circulifer tenellus
Spiroplasma citri movement into the intestines and salivary glands of its leafhopper vector, Circulifer tenellus
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DOI：
10.1094/phyto.1999.89.12.1144
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发表时间：
1999-12-01
期刊：
影响因子：
3.2
通讯作者：
Fletcher, J
中科院分区：
文献类型：
--
作者：
Kwon, MO;Wayadande, AC;Fletcher, J
柑橘螺原体（Spiroplasma citri）是柔膜菌纲（Mollicutes）中的一种螺旋状无壁原核生物，由甜菜叶蝉（Circulifer tenellus）传播。S.对叶蝉组织的入侵和细胞病理学效应。用透射电子显微镜观察了柑桔属植物的形态结构。所有8种细胞类型的主唾液腺，以及相邻的肌肉细胞和副唾液腺的细胞，由螺原体定殖。在中肠上皮和唾液腺细胞中，螺原体通常发生在膜结合的胞质小泡中，这些小泡通常位于细胞周边附近。在几个唾液腺细胞中。在膜包内也观察到螺旋体，这些膜包显然是由完整基膜下的质膜内陷形成的。这些观察结果与螺原体通过受体介导的内吞作用进入昆虫细胞是一致的。唾液细胞中螺原体感染的细胞病理学效应包括膜和基底层完整性的丧失，一些细胞中存在不规则的包涵体样结构，含有抗S标记的丝状物质的致密基质。citri抗体和内质网的明显紊乱。与健康肌细胞中紧密排列的纤维束相比，含螺原体的肌细胞中的纤维束出现弯曲和松散排列。这些症状可能会导致寿命和繁殖力的降低，这在以前的报道中已经出现。柑橘感染的C. tenellus。
Spiroplasma citri, a helical, wall-less prokaryote in the class Mollicutes, is transmitted by the beet leafhopper, Circulifer tenellus. Invasion of leafhopper tissues and cytopathological effects by S. citri were investigated by transmission electron microscopy. All eight cell types of the principle salivary glands, as well as the adjacent muscle cells and the cells of the accessory salivary glands, were colonized by the spiroplasmas. In both midgut epithelia and salivary gland cells, spiroplasmas usually occurred in membrane-bound cytoplasmic vesicles that often were located near the cell periphery. In several salivary gland cells. spiroplasmas were also observed within membranous packets apparently formed by invagination of the plasmalemma beneath intact basal lamina. These observations are consistent with spiroplasma entry into the insect cells by receptor-mediated endocytosis. Cytopathological effects of spiroplasma infection in salivary cells included loss of membrane and basal lamina integrity, presence in some cells of irregular inclusion-like structures containing dense matrices of filamentous material that labeled with anti-S. citri antibodies, and apparent disorganization of the endoplasmic reticulum. Compared to the tightly aligned fiber bundles in healthy muscle cells, bundles in spiroplasma-containing muscle cells appeared filamented and loosely arranged. Such symptoms could contribute to the reduction in longevity and fecundity that has been previously reported for S. citri-infected C. tenellus.