Effect of iron on the probiotic properties of the vaginal isolate Lactobacillus jensenii CECT 4306
Effect of iron on the probiotic properties of the vaginal isolate Lactobacillus jensenii CECT 4306
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DOI：
10.1099/mic.0.000044
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发表时间：
2015-04-01
期刊：
影响因子：
2.8
通讯作者：
Bermudez-Humaran, Luis G.
中科院分区：
文献类型：
--
作者：
Martin, Rebeca;Sanchez, Borja;Bermudez-Humaran, Luis G.
健康、育龄妇女的阴道微生物群以乳酸菌为主。作为一种防御机制，这些细菌产生H2O2来阻止不良微生物在阴道的定植。特别是，延森乳杆菌CECT 4306是H2O2的强生产者，并已被发现通过细胞外过氧化物酶的活性来保护自己免受这种化合物的杀菌作用。然而，这种过氧化物酶活性依赖于Fe3+的存在，由于月经分泌物，阴道粘膜中Fe3+的浓度升高。本研究的目的是评估Fe3+是否能够调节菌株4306的其他潜在益生菌特性。我们发现Fe3+增强了L. jensenii CECT 4306与粘蛋白、HT-29和HT-29 MTX细胞的粘附，此外，通过增加巨噬细胞分泌的IL-10/IL-12p70的比例来判断，Fe3+增强了L. jensenii CECT 4306对粘蛋白和HT-29 MTX细胞的粘附，并改善了抗炎特性。比较Fe3+存在和不存在时产生的总蛋白、分泌蛋白和表面蛋白，发现月光蛋白甘油醛-3-磷酸脱氢酶（GAPDH）的浓度有显著差异。综上所述，Fe3+似乎改善了L. jensenii CECT 4306的益生菌特性，未来对该菌株与阴道环境相互作用的研究可能会进一步揭示其益生菌潜力的不同方面。
The vaginal microbiota of healthy, fertile women is dominated by lactobacilli. As a defence mechanism, these bacteria produce H2O2 to discourage colonization of the vagina by undesirable micro-organisms. In particular, Lactobacillus jensenii CECT 4306 is a strong producer of H2O2 and has been found to protect itself from the bactericidal effects of this compound through the activity of extracellular peroxidases. However, this peroxidase activity is dependent on the presence of Fe3+, which is found in elevated concentrations in the vaginal mucosa as a consequence of the menstrual discharge. The aim of the present work was to evaluate whether Fe3+ is able to modulate other potential probiotic properties of strain 4306. We found that Fe3+ enhances the adhesion of L. jensenii CECT 4306 to mucin and to HT-29 and HT-29 MTX cells, and, in addition, improves the anti-inflammatory profile, as judged by an increase in the ratio of IL-10/IL-12p70 that were secreted by macrophages. A comparison of total, secreted and surface proteins produced in the presence and absence of Fe3+ revealed significant differences in the concentration of the moonlighting protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In conclusion, Fe3+ seems to improve the probiotic characteristics of L. jensenii CECT 4306, and future research of the interactions of this strain with its vaginal environment may reveal further information about different aspects of its probiotic potential.