Shewanella oneidensis in a lactate-fed pure-culture and a glucose-fed co-culture with Lactococcus lactis with an electrode as electron acceptor
Shewanella oneidensis in a lactate-fed pure-culture and a glucose-fed co-culture with Lactococcus lactis with an electrode as electron acceptor
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DOI：
10.1016/j.biortech.2010.10.033
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发表时间：
2011-02-01
影响因子：
11.4
通讯作者：
Angenent, Largus T.
中科院分区：
文献类型：
--
作者：
Rosenbaum, Miriam A.;Bar, Haim Y.;Angenent, Largus T.
采用混合微生物群落作为生物催化剂的生物电化学系统（BES）作为潜在的可再生能源、生物修复或生物传感装置越来越重要。虽然我们开始了解单个微生物物种如何与电极作为电子供体相互作用，但对社区中不同微生物物种之间的相互作用知之甚少：糖发酵细菌可以与当前生产微生物以中性，积极增强甚至负面影响的方式相互作用。在这里，我们比较希瓦氏菌oneidensis的生物电化学性能在纯培养和共培养的同型乳酸发酵乳酸乳球菌的条件下，是相关的常规BES操作。而S.单独的Oneidensis只能使用乳酸盐作为电流产生的电子供体，而共培养物能够以类似于17%的可比库仑效率将葡萄糖转化为电流。通过电化学分析和转录谱分析，我们发现BES性能和S. oneidensis的生理学无论是作为纯培养物还是共培养物生长都没有显著差异。因此，微生物以纯粹基于底物的（中性）关系一起工作。这些共培养实验代表了理解BES群落中微生物相互作用的重要一步，其目标是设计复杂的微生物群落，专门将目标底物转化为电能。(C)2010爱思唯尔有限公司版权所有。
Bioelectrochemical systems (BESs) employing mixed microbial communities as biocatalysts are gaining importance as potential renewable energy, bioremediation, or biosensing devices. While we are beginning to understand how individual microbial species interact with an electrode as electron donor, little is known about the interactions between different microbial species in a community: sugar fermenting bacteria can interact with current producing microbes in a fashion that is either neutral, positively enhancing, or even negatively affecting. Here, we compare the bioelectrochemical performance of Shewanella oneidensis in a pure-culture and in a co-culture with the homolactic acid fermenter Lactococcus lactis at conditions that are pertinent to conventional BES operation. While S. oneidensis alone can only use lactate as electron donor for current production, the co-culture is able to convert glucose into current with a comparable coulombic efficiency of similar to 17%. With (electro)-chemical analysis and transcription profiling, we found that the BES performance and S. oneidensis physiology were not significantly different whether grown as a pure- or co-culture. Thus, the microbes worked together in a purely substrate based (neutral) relationship. These co-culture experiments represent an important step in understanding microbial interactions in BES communities with the goal to design complex microbial communities, which specifically convert target substrates into electricity. (C) 2010 Elsevier Ltd. All rights reserved.