Periostin deposition in the stroma of invasive and intraductal neoplasms of the pancreas
Periostin deposition in the stroma of invasive and intraductal neoplasms of the pancreas
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DOI：
10.1038/modpathol.2008.77
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发表时间：
2008-08-01
期刊：
影响因子：
7.5
通讯作者：
Fukayama, Masashi
中科院分区：
文献类型：
--
作者：
Fukushima, Noriyoshi;Kikuchi, Yoshinao;Fukayama, Masashi
脱木质是胰腺导管腺癌的共同特征。该过程在宿主和肿瘤细胞之间复杂地相互作用。最近，通过转录组分析，骨膜素被鉴定为胰腺星状细胞中高度表达的基因。为了研究骨膜蛋白免疫沉积在胰腺肿瘤中的特征，我们进行了免疫组织化学和原位杂交，重点是肿瘤 - 质细胞相互作用。研究了81个手术切除的胰腺病变，包括35个胰腺导管腺癌，26个导管内乳头状粘膜肿瘤，11个粘液性囊性肿瘤和9个粘液性囊性肿瘤和9个慢性胰腺炎。在所有导管腺癌中，在免疫组织化学浸润性癌症周围的基质中都观察到骨膜素沉积。粘液性囊性肿瘤的细胞基质，称为“卵巢型”基质，没有显示骨膜蛋白沉积。在慢性胰腺炎中，骨膜蛋白的大多数染色模式都是围板和网状的。仅在原位杂交的基质细胞中检测到骨膜素基因表达。基于组织学等级，即腺瘤，非侵入性腺癌，具有显微镜浸润的腺癌和巨大的侵袭，将导管内乳头状粘膜肿瘤分为四组。与腺瘤相比，在导管内乳头粘液肿瘤的肿瘤中，腺癌骨膜蛋白沉积的频率和强度增加（p = 0.014）。此外，我们的结果表明，较高的骨膜素沉积频率与增殖上皮的“肠表型”的频率更高（P = 0.036）和层粘连蛋白-5 Gamma 2链链（P）（P
Desmoplasia is a common feature of infiltrating ductal adenocarcinoma of the pancreas. This process is intricately interacted between the host and neoplastic cells. Recently, by transcriptome analysis, periostin was identified as a significantly highly expressed gene in pancreatic stellate cells. To investigate the characteristics of periostin immunodeposition in pancreatic ductal neoplasms, we performed immunohistochemistry and in situ hybridization, focusing on tumor -stromal cells interactions. Eighty-one surgically resected pancreatic lesions, including 35 pancreatic ductal adenocarcinoma, 26 intraductal papillary-mucinous neoplasms, 11 mucinous cystic neoplasms and 9 chronic pancreatitis, were studied. In all ductal adenocarcinomas, periostin deposition was observed in the stroma around the infiltrating cancer on immunohistochemistry. Cellular stroma of mucinous cystic neoplasm, called 'ovarian-type' stroma, did not show periostin deposition. In chronic pancreatitis, most of the staining patterns of periostin were perilobular and meshwork-like. Periostin gene expression was detected solely in the stromal cells on in situ hybridization. Intraductal papillary-mucinous neoplasms were classified into four groups on the basis of the histological grade, namely, adenoma, non-invasive adenocarcinoma, adenocarcinoma with microscopical invasion and with macroscopically evident invasion. In intraductal papillary-mucinous neoplasm, periostin deposition in the periductal stroma increased in frequency and intensity in adenocarcinoma compared with adenomas (P = 0.014). Furthermore, our results showed that a higher frequency of periostin deposition was correlated with a higher frequency of ` intestinal phenotype' of proliferating epithelium (P = 0.036) and laminin-5 gamma 2 chain expression (P