ORAL VACCINATION WITH MICROENCAPSULED STRAIN 19 VACCINE CONFERS ENHANCED PROTECTION AGAINST BRUCELLA ABORTUS STRAIN 2308 CHALLENGE IN RED DEER (CERVUS ELAPHUS ELAPHUS)
ORAL VACCINATION WITH MICROENCAPSULED STRAIN 19 VACCINE CONFERS ENHANCED PROTECTION AGAINST BRUCELLA ABORTUS STRAIN 2308 CHALLENGE IN RED DEER (CERVUS ELAPHUS ELAPHUS)
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DOI：
10.7589/0090-3558-45.4.1021
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发表时间：
2009-10-01
影响因子：
1.3
通讯作者：
Rice-Ficht, Allison C.
中科院分区：
文献类型：
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作者：
Arenas-Gamboa, Angela M.;Ficht, Thomas A.;Rice-Ficht, Allison C.
美国大黄石地区(GYA)的野牛(Bison Bison)和麋鹿(Cervus Elaphus Nelsoni)感染了牛布鲁氏菌病的病原体--流产布鲁氏菌，它们是该病的野生动物宿主。最近，牛布鲁氏菌病在蒙大拿州、怀俄明州和爱达荷州从受感染的麋鹿传播到牛身上，导致它们失去了无布鲁氏菌病的状态。一种有效的布鲁氏菌疫苗，其递送系统适合野生动物，将是疾病预防和控制计划中的一个有价值的工具。我们在含有活疫苗的海藻酸盐微球组成的缓释载体中对菌株19(S19)进行了评估。在一项挑战研究中，使用马鹿(Cervus Elaffus Elaffus)作为麋鹿的模型，藻酸盐是一种自然产生的聚合物，与肝片吸虫的一种蛋白质结合。用卵黄蛋白B对S19进行微囊化。用1.5×10(10)集落形成单位(CFU)口服或皮下注射微囊化S19免疫梅花鹿。在整个研究过程中，每两个月分析一次嗡嗡声、口腔和细胞特征。免疫马鹿和未接种马鹿的对照组在免疫后1年用流产杆菌2308株1×10(9)CFU进行免疫攻击。与对照组相比，口服微囊化S19免疫马鹿的细菌组织负荷显著降低。这些数据首次表明，通过将常用疫苗与新型口服给药系统(如海藻酸盐-卵黄蛋白B微胶囊)相结合，可以实现对布鲁氏菌挑战的保护。该系统是向GYA野生动物提供有效布鲁氏菌疫苗的潜在改进。
Bison (Bison bison) and elk (Cervus elaphus nelsoni) in the Greater Yellowstone Area (GYA), USA, are infected with Brucella abortus, the causative agent of bovine brucellosis, and they serve as a wildlife reservoir for the disease. Bovine brucellosis recently has been transmitted from infected elk to cattle in Montana, Wyoming, and Idaho and has resulted in their loss of brucellosis-free status. An efficacious Brucella vaccine with a delivery system suitable for wildlife would be a valuable tool in a disease prevention and control program. We evaluated Strain 19 (S19) in a sustained release-vehicle consisting of alginate microspheres containing live vaccine. In a challenge study using red deer (Cervus elaphus elaphus) as a model for elk, alginate, a naturally occurring polymer combined with a protein of Fasciola hepatica. vitelline protein B was used to microencapsulate S19. lied deer were orally or subcutaneously immunized with 1.5X10(10) colony-forming units (CFUs) using microencapsulated S19. Hum oral and cellular profiles were analyzed bimonthly throughout the study. The vaccinated red deer and nonvaccinated controls were challenged 1 yr postimmunization conjunctivally with 1 X 10(9) CFUs of B. abortus strain 2308. Red deer vaccinated with oral microencapsulated S19 had a stafistically significant lower bacterial tissue load compared with controls. These data indicate for the first time that protection against Brucella-challenge can be achieved by combining a commonly used vaccine with a novel oral delivery system such as alginate-vitelline protein B microencapsulation. This system is a potential improvement for efficacious Brucella-vaccine delivery to wildlife in the GYA.