Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes
Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes
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DOI：
10.1530/rep-06-0341
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发表时间：
2007-09-01
期刊：
影响因子：
3.8
通讯作者：
Skinner, Michael K.
中科院分区：
文献类型：
--
作者：
Clement, Tracy M.;Anway, Matthew D.;Skinner, Michael K.
研究了性别决定和性腺分化过程中的基因表达谱，以发现新的潜在调控因子。用胚胎第13天（E13）、E14和E16大鼠的睾丸和卵巢进行微阵列分析，并在体外进行睾丸形态发生和有丝线索的E13睾丸器官培养。对E13和E16在雄性性腺发育中表达变化1.5倍的基因进行了选择性分析，得到了109个基因。对这109个对睾丸发育具有潜在重要意义的基因的表征表明，细胞骨架相关蛋白、细胞外基质因子和信号因子具有高度代表性。在整个发育时期（E13-E16），性别富集转录本在雄性中更为普遍，109个基因中有34个在性别决定期间具有睾丸富集表达。在卵巢中，El 3和El 6之间表达变化1.5倍的转录本总数与睾丸相似，但没有一个基因在发育期间同时在卵巢富集和调控。通过比较大鼠分析中改变的转录本，与先前发表的小鼠性腺性别决定研究中改变的转录本，鉴定了性别决定中保守的基因。通过比较小鼠和大鼠转录本的变化，鉴定出43个在性别决定和睾丸发育中具有物种保守性的基因。介绍了E13-E16大鼠睾丸和卵巢发育过程中的基因表达谱，并鉴定了参与性别决定和睾丸分化的候选基因。细胞通路分析未发现涉及多个候选基因的特定通路。然而，已确定的基因和基因网络影响许多细胞过程，包括细胞分化、增殖、局灶接触、RNA定位和发育。
Gene expression profiles during sex determination and gonadal differentiation were investigated to identify new potential regulatory factors. Embryonic day 13 (E13), E14, and E16 rat testes and ovaries were used for microarray analysis, as well as E13 testis organ cultures that undergo testis morphogenesis and develop serniniferous cords in vitro. A list of 109 genes resulted from a selective analysis for genes present in male gonadal development and with a 1.5-fold change in expression between E13 and E16. Characterization of these 109 genes potentially important for testis development revealed that cytoskeletal-associated proteins, extracellular matrix factors, and signaling factors were highly represented. Throughout the developmental period (E13-E16), sex-enriched transcripts were more prevalent in the male with 34 of the 109 genes having testis-enriched expression during sex determination. In ovaries, the total number of transcripts with a 1.5-fold change in expression between El 3 and El 6 was similar to the testis, but none of those genes were both ovary enriched and regulated during the developmental period. Genes conserved in sex determination were identified by comparing changing transcripts in the rat analysis herein, to transcripts altered in previously published mouse studies of gonadal sex determination. A comparison of changing mouse and rat transcripts identified 43 genes with species conservation in sex determination and testis development. Profiles of gene expression during E13-E16 rat testis and ovary development are presented and candidate genes for involvement in sex determination and testis differentiation are identified. Analysis of cellular pathways did not reveal any specific pathways involving multiple candidate genes. However, the genes and gene network identified influence numerous cellular processes with cellular differentiation, proliferation, focal contact, RNA localization, and development being predominant.