Gene expression during formation of earlywood and latewood in loblolly pine: Expression profiles of 350 genes
Gene expression during formation of earlywood and latewood in loblolly pine: Expression profiles of 350 genes
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DOI：
10.1055/s-2004-830383
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发表时间：
2004-11-01
期刊：
影响因子：
3.9
通讯作者：
Sederoff, R
中科院分区：
文献类型：
--
作者：
Egertsdotter, U;van Zyl, LM;Sederoff, R
树木木材形成的自然变异性提供了将转录谱与木材特性相关联的机会。利用基因芯片技术研究了火炬松次生木质部发育过程中转录本的丰度。这些cdna是从已测序和注释的75000条est中选择的（http://web.ahc.umn.edu/biodata/ nsfpine/）。细胞壁厚度和气候数据与生长季节不同时间点的早木和晚木形成有关。71个est在早木或晚木中优先表达，其中23个基因与GenBank中的基因没有显著的相似性。7个与木质素合成有关的基因在晚木中优先表达。这些研究初步揭示了与木材形成过程有关的一些基因表达模式的变化。
The natural variability of wood formation in trees affords opportunities to correlate transcript profiles with the resulting wood properties. We have used cDNA microarrays to study transcript abundance in developing secondary xylem of loblolly pine (Pinus taeda) over a growing season. The cDNAs were selected from a collection of 75000 ESTs that have been sequenced and annotated (http://web.ahc.umn.edu/biodata/ nsfpine/). Cell wall thickness and climatic data were related to earlywood and latewood formation at different time points during the growing season. Seventy-one ESTs showed preferential expression in earlywood or latewood, including 23 genes with no significant similarity to genes in GenBank. Seven genes involved in lignin synthesis were preferentially expressed in latewood. The studies have provided initial insights into the variation of expression patterns of some of the genes related to the wood formation process.