Hepatoprotective effects of Yi Guan Jian, an herbal medicine, in rats with dimethylnitrosamine-induced liver fibrosis
Hepatoprotective effects of Yi Guan Jian, an herbal medicine, in rats with dimethylnitrosamine-induced liver fibrosis
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DOI：
10.1016/j.jep.2011.02.013
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发表时间：
2011-04-12
影响因子：
5.4
通讯作者：
Chen, Chuan-Mu
中科院分区：
文献类型：
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作者：
Lin, Hung-Jen;Chen, Jiun-Yu;Chen, Chuan-Mu
研究目的：中药一贯煎治疗肝纤维化已有较长的临床应用历史，但其作用机制尚不清楚。本研究旨在评估二甲基亚硝胺（DMN）诱导的大鼠肝纤维化中纤维化途径的参与。材料和方法：通过DMN注射（10 mg/kg，i. p.，每周连续给药三天）。口服给予YGJ（每天1.8g/kg，通过胃管灌胃，持续两周）。对肝脏样品进行组织学和蛋白质印迹研究。结果：YGJ能显著抑制大鼠体重减轻和肝脏DMN损伤，抑制血清谷草转氨酶（GOT）和谷丙转氨酶（GPT）的升高。口服给药的YGJ提取物显着减少胶原蛋白α 1-I，TIMP-1，和α-SMA在肝tissue.Conclusions的积累：两者合计，这些研究结果表明，YGJ中药对DMN诱导的肝损伤表现出肝保护和抗纤维化作用。我们的数据表明，YGJ可能是有用的逆转肝纤维化的发展。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Aims of the study: Yi Guan Jian (YGJ) has long been employed clinically to treat liver fibrosis in traditional Chinese Medicine but the mechanism underlying the regulation has not been clarified in detail. The present investigation was designed to assess the involvement of the fibrosis pathway in dimethylnitrosamine (DMN)-induced liver fibrosis in rats.Materials and methods: Liver fibrosis was induced by DMN injection (10 mg/kg, i.p., given three consecutive days each week) following 4 weeks. YGJ was oral administered (1.8 g/kg daily via gastrogavage for two weeks). Liver sample were subjected to histological and western blot studies. For evaluation of hepatic fibrosis-related factors, collagen alpha 1-I tissue inhibitor of metalloproteinase-1 (TIMP-1), and alpha-smooth muscle actin (alpha-SMA) mRNA and protein levels were analyzed.Results: YGJ remarkably prevented body weight loss and DMN damage in the liver, and it inhibited the elevation of serum glutamate oxaloacetate transaminase (GOT), and glutamic pyruvic transaminase (GPT). Oral administration of YGJ extract significantly reduced the accumulation of collagen alpha 1-I, TIMP-1, and alpha-SMA in liver tissues.Conclusions: Taken together, these findings indicate that the YGJ Chinese herb showed hepatoprotective and anti-fibrogenic effects against DMN-induced hepatic injury. Our data suggest that the YGJ may be useful in reversing the development of hepatic fibrosis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.