Synthesis, morphology and antifungal activity of nano-particulated amphotericin-B, ketoconazole and thymoquinone against Candida albicans yeasts and Candida biofilm
Synthesis, morphology and antifungal activity of nano-particulated amphotericin-B, ketoconazole and thymoquinone against Candida albicans yeasts and Candida biofilm
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DOI：
10.1080/10934529.2015.975042
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发表时间：
2015-01-28
影响因子：
2.1
通讯作者：
Ali, Ashraf
中科院分区：
文献类型：
--
作者：
Randhawa, Mohammad A.;Gondal, Mohammed A.;Ali, Ashraf
In the current study, nano-particulated drugs-Amphotericin-B, Ketoconazole and Thymoquinone (an active ingredient of Nigella sativa)-were prepared using the ball milling technique, and their particle sizes were examined by transmission electron microscopy (TEM) and using a particle size analyzer. The grain sizes of the prepared compounds were found in between 5 to 20nm, and exhibited quasi-spherical morphology. The antifungal activity of each nano-particulated drug was investigated in vitro against Candida albicans yeasts and Candida biofilm, and compared with their micro-structured conventional forms. Nano-sized drugs were found to be two to four times more effective in disinfecting both the Candida yeasts and Candida biofilm. The study is a first of its kind as nano-forms of drugs have not been studied against Candida and Candida biofilm before. Further investigations are required for the determination of the clinical significance of the nano-formulation of antifungal substances.
