Two independently regulated secretory systems within the acini of the submandibular gland of the perinatal rat.
Two independently regulated secretory systems within the acini of the submandibular gland of the perinatal rat.
复制标题
围产期大鼠下颌下腺腺泡内有两个独立调节的分泌系统。
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Redman Rs
中科院分区：
文献类型：
--
作者：
Ball Wd;Redman Rs
大鼠颌下腺在围产期腺泡中经历一个短暂的分化阶段。两种形态上不同的细胞类型（I型和III型）出现并分泌几种细胞学上可识别的蛋白质。为了检查这些蛋白质的分泌的调节和它们与两种细胞类型的分泌的关联，将4天大的腺体与分泌激动剂和拮抗剂一起孵育，并评价培养基中分泌的蛋白质的培养物，并对细胞的脱粒进行形态学测定。刺激腺体β-肾上腺素能受体激动剂导致分泌的三种蛋白质，A，B1和B2，在Rf 0.26，0.36和0.40在本地，阴离子凝胶在pH 8.3。胆碱能刺激引起的外观不同的蛋白质，C，在Rf 0.12，但只有轻微的分泌A，B1和B2。在β-肾上腺素能和胆碱能刺激下，另一种蛋白质D在起源附近被观察到。蛋白质的部分纯化通过凝胶色谱法，然后通过制备型IEF（等电点聚焦）来实现。分别在等电pH 4.5、5.4和5.1下回收蛋白A、B1和B2。IEF组分和分泌培养基的SDS凝胶显示A、B1、B2、C和D的Mr分别为25 K、26 K、27 K、97 K和大于150 K。β-肾上腺素能刺激仅引起I型细胞的脱粒，胆碱能刺激仅引起III型细胞的脱粒。我们提出存在两个独立调节的分泌系统在围产期腺泡，与I型细胞分泌蛋白C刺激胆碱能受体，和III型细胞分泌A，B1，和B2通过β-肾上腺素能受体。在任一刺激下，蛋白D被分泌，因此它可以在分泌过程中发挥一般功能。
: Rat submandibular glands undergo a transient stage of differentiation in the perinatal acini. Two morphologically distinct cell types (Type I and Type III) appear and secrete several electrophoretically identifiable proteins. To examine the regulation of the secretion of these proteins and their association with secretion by the two cell types, 4-day-old glands were incubated with secretory agonists and antagonists, and the cultures were evaluated for secreted proteins in the medium and morphologically assayed degranulation of the cells. Stimulation of glands with beta-adrenergic agonists resulted in the secretion of three proteins, A, B1, and B2, at Rf 0.26, 0.36, and 0.40 in native, anionic gels at pH 8.3. Cholinergic stimulation caused the appearance of a different protein, C, at Rf 0.12, but only slight secretion of A, B1, and B2. With both beta-adrenergic and cholinergic stimulation, another protein, D, was seen near the origin. Partial purification of the proteins was effected by gel chromatography followed by preparative IEF (Isoelectric point focusing). Proteins A, B1, and B2 were recovered at isoelectric pH 4.5, 5.4, and 5.1 respectively. SDS gels of IEF fractions and secretion medium indicate Mr 25K, 26K, 27K, 97K, and greater than 150K for A, B1, B2, C, and D respectively. beta-Adrenergic stimulation caused degranulation only of Type I cells and cholinergic stimulation only of Type III cells. We propose the existence of two independently regulated secretory systems in the perinatal acini, with Type I cells secreting protein C on stimulation of cholinergic receptors, and Type III cells secreting A, B1, and B2 through beta-adrenergic receptors. With either stimulation, protein D is secreted, and thus it may serve a general function in the secretion process.