ELICITOR-INDUCED ETHYLENE BIOSYNTHESIS IN TOMATO CELLS - CHARACTERIZATION AND USE AS A BIOASSAY FOR ELICITOR ACTION
ELICITOR-INDUCED ETHYLENE BIOSYNTHESIS IN TOMATO CELLS - CHARACTERIZATION AND USE AS A BIOASSAY FOR ELICITOR ACTION
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DOI：
10.1104/pp.97.1.19
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发表时间：
1991-09-01
期刊：
影响因子：
7.4
通讯作者：
BOLLER, T
中科院分区：
文献类型：
--
作者：
FELIX, G;GROSSKOPF, DG;BOLLER, T
研究了从酵母抽提物中部分纯化的诱导子对番茄乙烯生物合成的诱导作用。细胞未受刺激的细胞在指数生长期间产生很少的乙烯，在静止期甚至更少。诱导子处理在指数生长的细胞中刺激乙烯生物合成10倍至20倍，在静止细胞中刺激乙烯生物合成100倍以上。活性的1-氨基环丙烷-1-羧酸（ACC）合酶，在体外测量，乙烯形成酶（EFE），在体内测量，强烈增加，响应激发子治疗。在指数生长期间，细胞含有大量ACC，并且诱导子主要通过诱导EFE刺激乙烯生物合成。在稳定期，细胞几乎不含ACC，诱导子刺激乙烯生物合成主要是通过其对ACC合酶活性的影响。虫草素不影响ACC合成酶活性的增加，但阻断EFE的活性，表明前者是转录后调节，后者是转录调节。去除激发子通过洗涤或灭活的生物素化的衍生物的激发子与抗生物素蛋白复合引起的ACC合酶活性的增加迅速停止，这表明刺激的持续存在是必要的响应。利用乙烯产生的诱导来测量激发子的量，发现在处理过程中激发子从孵育培养基中消失。
The induction of ethylene biosynthesis by an elicitor partially purified from yeast extract was studied in suspension-cultured tomato (Lycopersicon esculentum Mill.) cells. Unstimulated cells produced little ethylene during exponential growth and even less in stationary phase. Treatment with elicitor stimulated ethylene biosynthesis 10-fold to 20-fold in the exponentially growing cells and more than 100-fold in stationary cells. Activities of both 1-aminocyclopropane-1-carboxylate (ACC) synthase, measured in vitro, and ethylene-forming enzyme (EFE), measured in vivo, increased strongly in response to elicitor treatments. During exponential growth, cells contained large pools of ACC, and the elicitor stimulated ethylene biosynthesis primarily through induction of EFE. In the stationary phase, cells contained almost no ACC, and the elicitor stimulated ethylene biosynthesis primarily through its effect on ACC synthase activity. Cordycepin did not affect the increase in activity of ACC synthase but blocked that of EFE, indicating that the former was posttranscriptionally regulated, the latter transcriptionally regulated. Removal of elicitor by washing or inactivation of a biotinylated derivative of the elicitor by complexation with avidin caused a rapid cessation of the increase in ACC synthase activity, suggesting that continuous presence of stimulus is necessary for the response. Using induction of ethylene production to measure amounts of elicitor, it was found that the elicitor disappeared from the incubation medium during the course of the treatment.