Genome-wide Screening of Regulators of Catalase Expression ROLE OF A TRANSCRIPTION COMPLEX AND HISTONE AND tRNA MODIFICATION COMPLEXES ON ADAPTATION TO STRESS
Genome-wide Screening of Regulators of Catalase Expression ROLE OF A TRANSCRIPTION COMPLEX AND HISTONE AND tRNA MODIFICATION COMPLEXES ON ADAPTATION TO STRESS
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DOI：
10.1074/jbc.m115.696658
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发表时间：
2016-01-08
影响因子：
4.8
通讯作者：
Hidalgo, Elena
中科院分区：
文献类型：
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作者：
Garcia, Patricia;Encinar del Dedo, Javier;Hidalgo, Elena
为了响应环境信号，促分裂原活化蛋白激酶Sty 1驱动的信号级联激活数百个基因，以诱导裂变酵母中强大的抗应激细胞反应。因此，在压力施加Sty 1瞬时积累在细胞核中，它通过Atf 1转录因子上调转录。转录和翻译的几种调节因子已被确定为对氧化应激的整体反应很重要，例如Spt-Ada-Gcn 5-乙酰转移酶或Elongator复合物。为了鉴定这种大规模基因表达程序的新调节因子，我们使用了基于GFP的蛋白质报告基因，并使用流式细胞术筛选了裂殖酵母缺失集。我们发现，在过氧化物的威胁之前和之后，与GFP融合的过氧化氢酶的水平在数百种缺乏染色质修饰剂、转录复合物和翻译调节剂的菌株中发生改变。因此，转录延伸复合物Paf 1，组蛋白甲基化酶Set 1-COMPASS，和抑制相关的Trm 112二聚体都参与Ctt 1-GFP的完全表达和野生型过氧化物耐受性。
In response to environmental cues, the mitogen-activated protein kinase Sty1-driven signaling cascade activates hundreds of genes to induce a robust anti-stress cellular response in fission yeast. Thus, upon stress imposition Sty1 transiently accumulates in the nucleus where it up-regulates transcription through the Atf1 transcription factor. Several regulators of transcription and translation have been identified as important to mount an integral response to oxidative stress, such as the Spt-Ada-Gcn5-acetyl transferase or Elongator complexes, respectively. With the aim of identifying new regulators of this massive gene expression program, we have used a GFP-based protein reporter and screened a fission yeast deletion collection using flow cytometry. We find that the levels of catalase fused to GFP, both before and after a threat of peroxides, are altered in hundreds of strains lacking components of chromatin modifiers, transcription complexes, and modulators of translation. Thus, the transcription elongation complex Paf1, the histone methylase Set1-COMPASS, and the translation-related Trm112 dimers are all involved in full expression of Ctt1-GFP and in wild-type tolerance to peroxides.