Single-base Resolution Nucleosome Mapping on DNA Sequences
Single-base Resolution Nucleosome Mapping on DNA Sequences
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DOI：
10.1080/07391102.2010.10507347
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Trifonov, E. N.
中科院分区：
文献类型：
--
作者：
Gabdank, I.;Barash, D.;Trifonov, E. N.
从秀丽隐杆线虫的大型核小体DNA数据库中提取核小体DNA亲和性图谱，构建全长（116个二核苷酸位置）核小体DNA亲和性矩阵。该基质可用于序列上核小体的序列定向映射。通常可以预测给定核小体的几个可选位置。被核小体DNA周期的倍数分开。相应的计算机程序在最著名的精确定位核小体的实验实例上成功地进行了测试。计算映射的不确定性是+/- 1个基数。该程序放置在可公开访问的服务器上，可以应用于任何感兴趣的DNA序列。
Nucleosome DNA bendahility pattern extracted from large nucleosome DNA database of C. elegans is used for construction of full length (116 dinucleotide positions) nucleosome DNA bendahility matrix. The matrix can he used for sequence-directed mapping of the nucleosomes on the sequences. Several alternative positions for a given nucleosome are typically predicted. separated by multiples of nucleosome DNA period. The corresponding computer program is successfully tested on best known experimental examples of accurately positioned nucleosomes. The uncertainty of the computational mapping is +/- 1 base. The procedure is placed on publicly accessible server and can he applied to any DNA sequence of interest.