Amino acid sequence of rat submaxillary tonin reveals similarities to serine proteases
Amino acid sequence of rat submaxillary tonin reveals similarities to serine proteases
复制标题
大鼠颌下肌张力的氨基酸序列揭示了与丝氨酸蛋白酶的相似性
作者：
C. Lazure;R. Leduc;N. Seidah;G. Thibault;J. Genest;M. Chrétien
Tonin 1是一种从大鼠颌下腺分离的酯蛋白酶，是一种具有胰蛋白酶和胰凝乳蛋白酶样活性的丝氨酸蛋白酶。Tonin的底物特异性表明它不同于激肽释放酶，并且肯定不是类肾素酶或血管紧张素转化酶2。紧张素可以通过Phe-His键的断裂，由血管紧张素I、血管紧张素原和合成的肾素十四肽底物直接产生血管活性肽血管紧张素II。还发现它可裂解各种底物中的一些Phe和Arg键，如β-促脂素（β-LPH）、促肾上腺皮质激素（ACTH）、阿黑皮素原（POMC）3和P4物质。在这里，我们描述了完整的氨基酸序列的大鼠颌下腺，tonin。将219个氨基酸的序列与其他丝氨酸蛋白酶，特别是激肽释放酶、神经生长因子（NGF）的γ-亚基和最近描述的γ-肾素进行比较，揭示了广泛的相似性。更有趣的是，它还揭示了在丝氨酸蛋白酶活性位点三联体（Asp，His，Ser）中总是发现的Asp残基被Leu残基取代。这种不寻常的取代似乎不影响酶的蛋白水解活性。
Tonin1, an ester protease isolated from rat sub maxillary gland, is a serine protease with trypsin-and chymotrypsin-like activity. The substrate specificity of tonin shows that it differs from kallikreins and is definitely not a renin-like enzyme or an angiotensin-converting enzyme2. Tonin can produce directly the vasoactive peptide angiotensin II, from angiotensin I, angiotensinogen and the synthetic tetradecapeptide substrate of renin by cleavage of a Phe-His bond. It has also been found to cleave some Phe and Arg bonds in various substrates such as β-lipotropin (β-LPH), adrenocorticotropin (ACTH), proopiomelanocortin (POMC)3 and substance P4. Here we describe the complete amino acid sequence of rat submaxillary gland, tonin. Comparison of the sequence of 219 amino acids with other serine proteases, particularly kallikreins, γ-subunit of nerve growth factor (NGF) and the recently described γ-renin, reveals extensive similarities. More interestingly, it also reveals the substitution of an Asp residue always found in the serine protease active site triad (Asp, His, Ser) by a Leu residue. This unusual substitution does not seem to affect the proteolytic activity of the enzyme.
小鼠颌下腺7S神经生长因子γ亚基的氨基酸序列。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thomas,KA;Baglan,NC;Bradshaw,RA
通讯作者：
Bradshaw,RA
来自小鼠颌下腺的蛋白水解酶：自发裂解的部分序列和演示。
影响因子：
3.9
作者：
Schenkein,I;Franklin,EC;Frangione,B
通讯作者：
Frangione,B