Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges
Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges
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水稻 OsNAR2.1 与 OsNRT2.1、OsNRT2.2 和 OsNRT2.3a 硝酸盐转运蛋白相互作用，提供高浓度和低浓度范围的摄取
DOI：
10.1111/j.1365-3040.2011.02335.x
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发表时间：
2011-08-01
影响因子：
7.3
通讯作者：
Xu, Guohua
中科院分区：
文献类型：
--
作者：
Yan, Ming;Fan, Xiaorong;Xu, Guohua
植物吸收硝酸盐和铵作为主要氮源。尽管铵是厌氧淹水稻土中的主要形式，但有人提出，水稻和其他湿地植物可能会吸收根际铵硝化形成的大量硝酸盐。已在拟南芥中鉴定出硝酸盐转运的双组分系统，包括 NRT2 和伴侣蛋白（NAR2 或 NRT3.1）。我们报告了 NAR2 家族的另一个成员 OsNAR2.1 在水稻（Oryza sativa，ssp. Japonica，cv. Nipponbare）中的生理功能。 OsNAR2.1主要在根部表达，受硝酸盐诱导，受铵和一些氨基酸抑制。通过RNA干扰敲除OsNAR2.1同时抑制了osnar2.1突变体中OsNRT2.1、OsNRT2.2和OsNRT2.3a的表达。 OsNAR2.1 敲低使高亲和力和低亲和力硝酸盐转运均受到极大损害。酵母二杂交显示OsNAR2.1不仅与OsNRT2.1/OsNRT2.2相互作用，还与OsNRT2.3a相互作用。综上所述，数据表明 OsNAR2.1 在使工厂应对硝酸盐供应变化方面发挥着关键作用。
Plants take up both nitrate and ammonium as main nitrogen (N) sources. Although ammonium is the predominant form in anaerobic-flooded paddy soil, it has been proposed that rice and other wetland plants may take up significant amounts of nitrate formed by nitrification of ammonium in the rhizosphere. A two-component system for nitrate transport including NRT2s with a partner protein (NAR2 or NRT3.1) has been identified in Arabidopsis. We report the physiological function of another member of the NAR2 family, OsNAR2.1 in rice (Oryza sativa, ssp. Japonica, cv. Nipponbare). OsNAR2.1 was mainly expressed in roots and induced by nitrate and suppressed by ammonium and some amino acids. Knockdown of OsNAR2.1 by RNA interference synchronously suppressed expression of OsNRT2.1, OsNRT2.2 and OsNRT2.3a in the osnar2.1mutants. Both high- and low-affinity nitrate transports were greatly impaired by OsNAR2.1 knockdown. Yeast two hybridization showed that OsNAR2.1 not only interacted with OsNRT2.1/OsNRT2.2, but also with OsNRT2.3a. Taken together, the data demonstrate that OsNAR2.1 plays a key role in enabling the plant to cope with variable nitrate supply.