Tight junction disruption of blood-brain barrier in white matter lesions in chronic hypertensive rats
Tight junction disruption of blood-brain barrier in white matter lesions in chronic hypertensive rats
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DOI：
10.1097/wnr.0000000000000464
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发表时间：
2015-12-02
期刊：
影响因子：
1.7
通讯作者：
Sun, Jian
中科院分区：
文献类型：
--
作者：
Fan, Yuhua;Yang, Xian;Sun, Jian
紧密连接（TJs）是血脑屏障（BBB）最重要的结构。研究表明，白色病变（WML）的触发可能与BBB功能障碍有关，但很少有研究观察到TJ纵向的进行性变化。本研究应用透射电镜观察了易卒中肾血管性高血压大鼠TJ的超微结构。Western blotting检测TJ相关蛋白zonula occludens-1和occludin。结果表明，在易卒中肾血管性高血压大鼠中，WML的严重程度逐渐增加。高血压8周后，TJs逐渐破坏。闭合小带-1和occludin的水平也逐渐下降。提示长期高血压可导致血脑屏障TJs的逐渐破坏，并诱发慢性高血压大鼠的WML。版权所有（C）2015威科医疗集团All rights reserved.
Tight junctions (TJs) are the most important structure of the blood-brain barrier (BBB). Studies have shown that triggering of white matter lesions (WMLs) may be related to a BBB dysfunction, but rarely have studies observed the progressive changes in TJs longitudinally. In our present study, the ultrastructure of TJs was observed using a transmission electron microscope in Stroke-prone Renalvascular Hypertensive Rats. Western blotting was used to detect TJ-related proteins zonula occludens-1 and occludin. The results showed that in Stroke-prone Renalvascular Hypertensive Rats, the severity of WMLs increased gradually. TJs was destroyed gradually 8 weeks after hypertension. The levels of zonula occludens-1 and occludin also decreased gradually. These data suggested that long-term hypertension may contribute toward the gradual disruption of TJs of BBB and induce WMLs in chronic hypertensive rats. Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved.