Genetic Analysis of Variation in Gene Expression in Arabidopsis thaliana
Genetic Analysis of Variation in Gene Expression in Arabidopsis thaliana
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DOI：
10.1534/genetics.105.041509
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发表时间：
2005-11
期刊：
影响因子：
3.3
通讯作者：
M. Vuylsteke;F. V. van Eeuwijk;P. Van Hummelen;Martin Kuiper;M. Zabeau
中科院分区：
文献类型：
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作者：
M. Vuylsteke;F. V. van Eeuwijk;P. Van Hummelen;Martin Kuiper;M. Zabeau
在拟南芥中，确定表型差异材料之间基因组差异程度的重大努力正在进行中，但实际上对转录水平上的差异知之甚少。我们使用基因芯片检测了三个自交系和两对高度自交的拟南芥的正反交F1之间转录丰度的差异。基因表达的复合加性遗传效应是通过对哥伦比亚(COL)、兰德斯伯格直立(LER)和佛得角群岛(CVI)三个材料的配对比较来估计的。在COL和LER对中，27.0%的基因在表达上表现出加性遗传效应(α=0.001)，而在CVI与LER和COL配对中，分别为32.2%和37.5%。显著差异表达的倍数变化范围从32.45到1.10，差异通常为1.56倍。母本或父本的传播只影响几个基因，这表明在所分析的两个杂交中观察到的互惠效应是最小的。显性效应是通过与相应的中亲值进行比较来估计的。F_1代在表达水平上显性的基因百分比为6.4%~21.1%(α=0.001)。根据显性比的大小对这些数量的基因进行分解，平均有9%的基因表现出杂种优势。基因表达变异的遗传分析的进一步进展可能有助于更好地理解它在表型水平上影响数量性状变异的作用。
In Arabidopsis thaliana, significant efforts to determine the extent of genomic variation between phenotypically divergent accessions are under way, but virtually nothing is known about variation at the transcription level. We used microarrays to examine variation in transcript abundance among three inbred lines and two pairs of reciprocal F1 hybrids of the highly self-fertilizing species Arabidopsis. Composite additive genetic effects for gene expression were estimated from pairwise comparisons of the three accessions Columbia (Col), Landsberg erecta (Ler), and Cape Verde Islands (Cvi). For the pair Col and Ler, 27.0% of the 4876 genes exhibited additive genetic effects in their expression (α = 0.001) vs. 32.2 and 37.5% for Cvi with Ler and Col, respectively. Significant differential expression ranged from 32.45 down to 1.10 in fold change and typically differed by a factor of 1.56. Maternal or paternal transmission affected only a few genes, suggesting that the reciprocal effects observed in the two crosses analyzed were minimal. Dominance effects were estimated from the comparisons of hybrids with the corresponding midparent value. The percentage of genes showing dominance at the expression level in the F1 hybrids ranged from 6.4 to 21.1% (α = 0.001). Breakdown of these numbers of genes according to the magnitude of the dominance ratio revealed heterosis for expression for on average 9% of the genes. Further advances in the genetic analysis of gene expression variation may contribute to a better understanding of its role in affecting quantitative trait variation at the phenotypic level.