ZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016
ZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016
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从蚊子中分离出寨卡病毒：2016 年中国现场和实验室调查
DOI：
10.1007/s11427-017-9196-8
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发表时间：
2017-12-01
影响因子：
9.1
通讯作者：
Liang, Guodong
中科院分区：
文献类型：
--
作者：
Fu, Shihong;Song, Song;Liang, Guodong
2016年夏季在贵州德江进行了虫媒病毒的野外调查。共采集蚊类3属4种8,795只，蠓类1,300只。现场根据形态学对蚊子样本进行鉴定，并将合并的样本在实验室中研磨和离心。将上清液与蚊子组织培养细胞（C6/36）和哺乳动物细胞（BHK-21）孵育用于病毒分离。结果表明，中华按蚊占40%（3,540/8,795），致倦库蚊占30%（2,700/8,795），近毛阿蚊占29%（2,530/8,795）。此外，共获得8个病毒分离株，基因组测序显示，2个寨卡病毒（ZIKV）分别从致倦库蚊和近毛阿蚊分离; 3个日本脑炎病毒（JEV）从致倦库蚊分离; 2个班纳病毒（BAV）分别从致倦库蚊和中华按蚊分离; 1个浓核病毒（DNV）从致倦库蚊分离。从致倦库蚊（Culex pipiens quinquefasciatus）和近端阿蚊（Armigeres subbalbeatus）中分离的ZIKV代表了中国大陆的第一个ZIKV分离株。这一发现为中国ZIKV的预防和控制提出了新的挑战，并促进了这一全球性问题的国际合作。
A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells (C6/36) and mammalian cells (BHK-21) for virus isolation. The results indicated that 40% (3,540/8,795) wereAnopheles sinensis, 30% (2,700/8,795) wereCulex pipiens quinquefasciatus, and 29% (2,530/8,795) wereArmigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses (ZIKVs) isolated fromCulex pipiens quinquefasciatusandArmigeres subbalbeatus, respectively; three Japanese encephalitis viruses (JEVs) isolated fromCulex pipiens quinquefasciatus; two Banna viruses (BAVs) isolated fromCulex pipiens quinquefasciatusandAnopheles sinensis, respectively; and one densovirus (DNV) isolated fromCulex pipiens quinquefasciatus. The ZIKVs isolated from theCulex pipiens quinquefasciatusandArmigeres subbalbeatusmosquitoes represent the first ZIKV isolates in mainland China. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue.