The effects of copper and benzo[a]pyrene on retinoids and reproduction in zebrafish
The effects of copper and benzo[a]pyrene on retinoids and reproduction in zebrafish
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DOI：
10.1016/j.aquatox.2007.03.001
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发表时间：
2007-05-31
影响因子：
4.5
通讯作者：
Van der Kraak, Glen
中科院分区：
文献类型：
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作者：
Alsop, Derek;Brown, Scott;Van der Kraak, Glen
本研究探讨是否存在有毒物质暴露，类维生素A和鱼类繁殖之间的联系。给斑马鱼喂食对照饮食（8.1 μ g Cu/g饮食，0 μ g苯并[a]芘/g饮食）或含有升高的铜（100 μ g、500 μ g和1000 μ g Cu/g饮食）或苯并[a]芘（B[a]P; 30和150 μ g B[a]P/g饮食）的饮食260天。添加毒物的饮食不影响生长或死亡率。虽然在实验过程中，对照组斑马鱼的全身类维生素A水平下降，但暴露于Cu或B[a]P 200天或更长时间的雌性经历了视黄酯（45-100%耗尽）和视网膜（B[a] P喂养的鱼中45%耗尽）的额外损失。尽管类维生素A减少，但Cu和B[a]P在产卵数量、受精率或卵视网膜含量方面不影响生殖（相反，在来自B[a] P喂养的鱼的卵中视网膜增加55-65%）。在任何处理的受精后36 h胚胎中均未观察到明显畸形。看来，虽然内部类维生素A储存耗尽的成年人，饮食类维生素A足以满足视网膜沉积在鸡蛋和视黄酸合成的日常需求。这项研究表明，雌性斑马鱼中的类维生素A水平对Cu和B[a]P敏感，是长期暴露的良好指标。它还揭示了鱼类类维生素A系统的弹性和饮食对毒理学反应的重要性。特别是，饮食类维生素A似乎支持正常生殖的情况下，内部维生素A存储。(c)2007 Elsevier B. V.保留所有权利。
This study examines whether a link exists between toxicant exposure, retinoids and reproduction in fish. Zebrafish were fed a control diet (8.1 mu g Cu/g diet, 0 mu g benzo[a]pyrene/g diet) or diets containing elevated copper (100 mu g, 500 mu g and 1000 mu g Cu/g diet) or benzo[a]pyrene (B[a]P; 30 and 150 mu g B[a]P/g diet) for 260 days. Toxicant-supplemented diets did not affect growth or mortality rates. While whole body retinoid levels in control zebrafish decreased during the experiment, females exposed to Cu or B[a]P for 200 days or more experienced additional losses of retinyl esters (45-100% depleted) and retinal (45% depleted in B[a]P-fed fish). Despite the reduced retinoids, Cu and B[a]P did not effect reproduction with respect to the number of eggs spawned, fertilization rates or egg retinal content (retinal was instead increased 55-65% in eggs from B[a]P-fed fish). There were no apparent deformities observed in 36 h post fertilization embryos from any treatment. It appears that although internal retinoid stores were depleted in adults, dietary retinoids were sufficient to meet the daily requirement for retinal deposition in the eggs and retinoic acid synthesis. This study has shown that retinoid levels in female zebrafish are sensitive to Cu and B[a]P, and are a good indicator of long-term exposure. It also brings to light the resiliency of the retinoid system in fish and the importance of the diet on the toxicological response. Specifically that dietary retinoids appear to support normal reproduction in the absence of internal retinoid stores. (c) 2007 Elsevier B.V. All rights reserved.