CHROMOSOME REPLICATION DURING DEVELOPMENT IN CAULOBACTER-CRESCENTUS
CHROMOSOME REPLICATION DURING DEVELOPMENT IN CAULOBACTER-CRESCENTUS
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DOI：
10.1016/0022-2836(72)90090-3
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发表时间：
1972-01-01
影响因子：
5.6
通讯作者：
NEWTON, A
中科院分区：
文献类型：
--
作者：
DEGNEN, ST;NEWTON, A
用一种新的细胞同步化方法测定了二型细菌新月柄杆菌的DNA复制和发育模式。特征DNA周期被确定为两种细胞类型。群集细胞周期由G1期、S期和G2期组成，分别为65、85和30 min;柄状细胞周期由S期和G2期组成，分别为90和30 min。因此，分裂时产生的两种细胞类型在不同的时间启动DNA合成：在有柄细胞中，染色体复制立即开始，而在群集细胞中，复制的开始延迟约65分钟。由于DNA复制中的合成前间隙对应于蜂群细胞形成柄所需的时间，因此DNA合成仅是有柄形式的C的特征。crescentus。结果表明，可能有一个结构的要求，在这些细菌中的DNA复制的启动，并在柄细胞的这种要求已得到满足，在分裂，而在蜂群细胞进一步发展是必需的。
Patterns of DNA replication and development have been determined in the dimorphic bacteriumCaulobacter crescentususing a new method of cell synchrony. Characteristic DNA cycles were identified for the two cell types. The swarmer cell cycle is composed of G1, S and G2periods of 65, 85 and 30 minutes, respectively, and the stalked cell cycle is composed of S and G2periods of 90 and 30 minutes. Thus, the two cell types produced at division initiate DNA synthesis at different times: in the stalked cell chromosome replication begins immediately, while in the swarmer cell the onset of replication is delayed for approximately 65 minutes. Since the pre-synthetic gap in DNA replication corresponds to the time required for stalk formation by the swarmer cell, DNA synthesis is characteristic only of the stalked form ofC. crescentus. The results suggest that there may be a structural requirement for initiation of DNA replication in these bacteria, and that in the stalked cell this requirement for initiation has been satisfied at division, while in the swarmer cell further development is required.