Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity
Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity
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DOI：
10.1111/j.1365-313x.2009.04000.x
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发表时间：
2009-12-01
期刊：
影响因子：
7.2
通讯作者：
Job, Dominique
中科院分区：
文献类型：
--
作者：
Tanou, Georgia;Job, Claudette;Job, Dominique
过氧化氢（H2 O2）和一氧化氮（中心点NO）是激活植物防御生物或非生物胁迫的信号转导途径中的关键活性物质。本文研究了预处理对柑桔（Citrus aurantium L.）用这两种分子中的任何一种对植物适应盐度的影响，并表明两种预处理都强烈地降低了伴随这种胁迫的有害表型和生理效应。蛋白质组学分析揭示了85个叶蛋白，在直接暴露于盐胁迫的植物中经历了显着的定量变化。这些变化的很大一部分没有观察到与盐胁迫植物预处理过H2 O2或硝普钠（SNP;中心点NO释放化学品）。我们还确定了几种蛋白质发生变化，无论是在其氧化（羰基化; 40蛋白质）和/或S-亚硝基化（49蛋白质）的状态，在响应盐度胁迫。H2 O2和SNP预处理盐胁迫前减轻盐诱导的蛋白质羰基化和转移的积累水平的叶片S-亚硝基化蛋白质的非胁迫对照植物。总之，结果表明H2 O2-和中心点NO-信号通路之间的重叠，在适应盐度，并建议，氧化和S-亚硝基化模式的叶蛋白是特定的分子签名柑橘植物活力在压力条件下。
P>Hydrogen peroxide (H2O2) and nitric oxide (center dot NO) are key reactive species in signal transduction pathways leading to activation of plant defense against biotic or abiotic stress. Here, we investigated the effect of pre-treating citrus plants (Citrus aurantium L.) with either of these two molecules on plant acclimation to salinity and show that both pre-treatments strongly reduced the detrimental phenotypical and physiological effects accompanying this stress. A proteomic analysis disclosed 85 leaf proteins that underwent significant quantitative variations in plants directly exposed to salt stress. A large part of these changes was not observed with salt-stressed plants pre-treated with either H2O2 or sodium nitroprusside (SNP; a center dot NO-releasing chemical). We also identified several proteins undergoing changes either in their oxidation (carbonylation; 40 proteins) and/or S-nitrosylation (49 proteins) status in response to salinity stress. Both H2O2 and SNP pre-treatments before salinity stress alleviated salinity-induced protein carbonylation and shifted the accumulation levels of leaf S-nitrosylated proteins to those of unstressed control plants. Altogether, the results indicate an overlap between H2O2- and center dot NO-signaling pathways in acclimation to salinity and suggest that the oxidation and S-nitrosylation patterns of leaf proteins are specific molecular signatures of citrus plant vigour under stressful conditions.