Detection of Varicella-Zoster Virus DNA in 414 Human Trigeminal Ganglia From Cadavers by the Polymerase Chain Reaction: A Comparison of the Detection Rate of Varicella-Zoster Virus and Herpes Simplex Virus Type 1
Detection of Varicella-Zoster Virus DNA in 414 Human Trigeminal Ganglia From Cadavers by the Polymerase Chain Reaction: A Comparison of the Detection Rate of Varicella-Zoster Virus and Herpes Simplex Virus Type 1
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DOI：
10.1002/jmv.21687
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发表时间：
2010-02-01
影响因子：
12.7
通讯作者：
Iwase, Hirotaro
中科院分区：
文献类型：
--
作者：
Inoue, Hiroyuki;Motani-Saitoh, Hisako;Iwase, Hirotaro
调查水痘带状疱疹病毒（VZV）具有重要的流行病学意义，确定其在人类三叉神经节的流行率对提供监测资料具有重要意义。迄今为止，三叉神经节VZV检测的研究使用了从相对有限数量的尸体中获得的标本。本研究采用聚合酶链反应（PCR）方法，从随机选取的207具尸体的414份三叉神经节样本中检测VZV和单纯疱疹病毒1型（HSV-1） DNA。检查检出率，确定左右三叉神经节、男女、年龄组之间的检出率是否有显著差异。VZV阳性率与HSV-1阳性率之间存在相关性。三叉神经节391例（94.4%）和尸体201例（97.1%）检出VZV DNA，其中男性121/124，女性80/83。其中，男性72/124例，女性62/83例，尸体134例（64.7%）检出HSV-1 DNA。两种病毒在左右三叉神经节和男女之间的检出率均无显著差异。年龄与HSV-1阳性呈显著相关，但与VZV无显著相关。所有134具HSV-1阳性的尸体也呈VZV阳性。VZV和HSV-1潜伏于双侧三叉神经节，不受性别影响。HSV-1阳性率与vzv阳性率呈正相关，年龄越高，HSV-1阳性率越高。中华检验医学杂志，32(2):349 -349,2010。(C) 2009 Wiley-Liss, Inc。
Investigation of varicella-zoster virus (VZV) is important epidemiologically, and determination of its prevalence rate in human trigeminal ganglia is important to provide surveillance data. To date, studies on VZV detection in trigeminal ganglia have used specimens obtained from a relatively limited number of cadavers. This study attempted to detect VZV DNA as well as Herpes simplex virus type 1 (HSV-1) DNA by the polymerase chain reaction (PCR) from 414 samples of trigeminal ganglia obtained from 207 cadavers selected at random. The detection rate was examined to determine whether there were significant differences in the positive rate between the left and right trigeminal ganglia, males and females, and among age groups. A relationship was found between the positive rates for VZV and HSV-1. VZV DNA was detected in 391 of the trigeminal ganglia (94.4%) and 201 of the cadavers (97.1%) in 121/124 males and 80/83 females. HSV-1 DNA was detected in 251 of the samples (60.6%) and 134 of the cadavers (64.7%) in 72/124 males and 62/83 females. There was no significant difference for either virus in the detection rates between the left and right trigeminal ganglia and males and females. Age and positivity for HSV-1, but not VZV, showed a significant relationship. All 134 cadavers positive for HSV-1 were also positive for VZV. VZV and HSV-1 become latent in bilateral trigeminal ganglia, and are not affected by gender. The prevalence of HSV-1 was greater in advanced age, and the HSV-1-positive rate was correlated with the VZV-positive rate. J. Med. Virol. 82: 345-349, 2010. (C) 2009 Wiley-Liss, Inc.